RNA干扰对LCDV-cn在牙鲆体内复制的影响  

Effect of RNA Interference on the Replication of LCDV-cn in Paralichthys olivaceus

作　　者：闫秀英[1] 邢明清[2] 郑风荣[3] 孙修勤[3] 简纪常[1] 吴灶和[4] 

机构地区：[1]广东海洋大学广东省水产经济动物病原生物学及流行病学重点实验室,广东湛江524025 [2]青岛市市立医院,山东青岛266011 [3]国家海洋局第一海洋研究所,山东青岛266061 [4]仲恺农业工程学院,广东广州510225

出　　处：《广东海洋大学学报》2013年第1期38-43,共6页Journal of Guangdong Ocean University

基　　金：国家高技术研究发展计划项目(2006AA100309)

摘　　要：海水养殖生物病毒病是世界性难题,淋巴囊肿病毒中国分离株(LCDV-cn)是牙鲆(Paralichthys olivaceus)淋巴囊肿病的病原。以mcp基因为靶基因,设计siRNA-mcp1、siRNA-mcp2、siRNA-mcp3等3条siRNA序列,研究RNA干扰(RNAi)对淋巴囊肿病毒在牙鲆体内复制的影响。结果表明,牙鲆体内注入siRNA-mcp1和siRNA-mcp3后,mcp基因的表达有所下调;统计分析表明,注入siRNA-mcp1后,mcp基因的表达下调明显,具有显著性差异(p<0.05),注入siRNA-mcp2、siRNA-mcp3后,mcp基因的表达下调不明显,差异不显著(p>0.05)。本实验中初步实现了RNAi对LCDV-cn复制的抑制作用。Lymphocystis disease virus- Chinese isolate （LCDV-cn） is the pathogen of lymphocystis disease in Paralichthys olivaceus, causing a huge loss in flounder aquaculture. Controlling viral infection in aquiculture is a technological challenge worldwide, and RNAi could be a way of preventing viral infection of cultured animals. In this study, the mcp gene as a target gene, three siRNA sequences （siRNA-mcpl, siRNA-mcp2 and siRNA-.mcp3） were designed and used to study the effect of RNA interference （RNAi） on the replication of LCDV-cn in the body of Paralichthys olivaceus. The experimental results showed that the expression of the mcp gene had the down-regulation after siRNA-mcpl and siRNA-mcp3 had been injected in the body of Paralichthys olivaceus. Statistical analysis showed that the down expression of the mcp gene was significantly different （p 〈 0.05） after the injection of siRNA-mcpl in the body of Paralichthys olivaceus, but did not occur （p 〉 0.05） after the injection of siRNA-mcp2 and siRNA-mcp3. The preliminary realization of the inhibition of RNAi on the replication of LCDV-cn had been acquired in this study.

关 键 词：淋巴囊肿病毒中国分离株(LCDV-cn) mcp基因 RNA干扰 

分 类 号：Q939.4[生物学—微生物学]