Characterization of erythromycin-resistant Streptococcus pneumoniae isolates causing invasive diseases in Chinese children  

作　　者：MA Xiang YAO Kai-hu XIE Gui-lin ZHENG YUE-jie WANG Chuan-qing SHANG Yun-xiao WANG Hui-yun WAN Li-Ya LIU Lan LI Chang-chong JI Wei XU Xi-wei WANG Ya-ting XU Pei-ru YU Sang-jie YANG Yong-hong 

机构地区：[1]Key Laboratory of Major Diseases in Children and the NationalKey Discipline of PediatricsMinistry of Education, Beijing Pediatrics Research Institute,Beijing Children's Hospital, Beijing 10045, China [2]Key Laboratory of Major Diseases in Children and the NationalKey Discipline of PediatricsLanzhou Institute of Biological Products, Lanzhou, Gansu 730030,China [3]Key Laboratory of Major Diseases in Children and the NationalKey Discipline of PediatricsShenzhen Children's Hospital, Shenzhen, Guangdong 518000,China [4]Key Laboratory of Major Diseases in Children and the NationalKey Discipline of PediatricsPediatric Hospital of Fudan University, Shanghai 200102, China [5]Key Laboratory of Major Diseases in Children and the NationalKey Discipline of PediatricsShengjing Hospital Affiliated to China Medical University,Shenyang, Liaoning 110100, China [6]Key Laboratory of Major Diseases in Children and the NationalKey Discipline of Pediatrics Nanjing Children's Hospital, Nanjing, Jiangsu 210000, China [7]Key Laboratory of Major Diseases in Children and the NationalKey Discipline of Pediatrics Tianji-n Children's Hospital, Tianjin 300000, China [8]Key Laboratory of Major Diseases in Children and the NationalKey Discipline of Pediatrics Chongqing Children's Hospital Affiliated to Chongqing MedicalUniversity, Chongqing 401600, China [9]Key Laboratory of Major Diseases in Children and the NationalKey Discipline of Pediatrics Yuying iEhildren's Hospital Affiliated to Wenzhou MedicalCollege, Wenzhou, Zhejiang 325500, China [10]Key Laboratory of Major Diseases in Children and the NationalKey Discipline of Pediatrics Yuying Children's Hospital Affiliated to Wenzhou MedicalCollege, Wenzhou, Zhejiang 325500, ChinaSoochow University Pediatric Hospital, Suzhou, Jiangsu 215400,China [11]Key Laboratory of Major Diseases in Children and the NationalKey Discipline of Pediatrics Anhui Medical University Affiliated Hospital, Hefei, Anhui230100, China [12]Key Laboratory of Major Diseases in Children and the NationalKey Discipline of Pedi

出　　处：《Chinese Medical Journal》2013年第8期1522-1527,共6页中华医学杂志（英文版）

摘　　要：Background Erythromycin-resistant Streptococcus pneumoniae isolates that causing invasive pneumococcal diseases （IPD） in Chinese children remain uncharacterized. This study aims to identify the resistance genes associated with erythromycin resistance and to determine the genetic relationships of IPD isolates in Chinese children. Methods A total of 171 S. pneumoniae strains were isolated from 11 medical centers in China from 2006 to 2008. All the isolates were characterized via serotyping and antibiotic susceptibility determination. The erythromycin-resistant isolates were further characterized via ermB and mefA gene detection, multi-locus sequence typing analysis, and pulsed-field gel electrophoresis. Results A total of 164 （95.9%） isolates showed resistance to erythremycin, of which 162 strains with high high-level resistance （MIC 〉 256 pg/ml）. A total of 104 （63.4%） isolates carry the ermB gene alone, whereas 59 （36.0%） harbor both ermB and mefA genes. Of the 59 strains, 54 were of serotypes 19A and 19F and were identified as highly clonal and related to the TaiwanlgF-14 clone. Conclusions The erythromycin resistance rate in IPD isolates is significantly high and is predominantly mediated by the ermB aene. Isolates that carrv both ermB and mefA Qenes are oredominantlv of serotvoes 19A and 19EBackground Erythromycin-resistant Streptococcus pneumoniae isolates that causing invasive pneumococcal diseases （IPD） in Chinese children remain uncharacterized. This study aims to identify the resistance genes associated with erythromycin resistance and to determine the genetic relationships of IPD isolates in Chinese children. Methods A total of 171 S. pneumoniae strains were isolated from 11 medical centers in China from 2006 to 2008. All the isolates were characterized via serotyping and antibiotic susceptibility determination. The erythromycin-resistant isolates were further characterized via ermB and mefA gene detection, multi-locus sequence typing analysis, and pulsed-field gel electrophoresis. Results A total of 164 （95.9%） isolates showed resistance to erythremycin, of which 162 strains with high high-level resistance （MIC 〉 256 pg/ml）. A total of 104 （63.4%） isolates carry the ermB gene alone, whereas 59 （36.0%） harbor both ermB and mefA genes. Of the 59 strains, 54 were of serotypes 19A and 19F and were identified as highly clonal and related to the TaiwanlgF-14 clone. Conclusions The erythromycin resistance rate in IPD isolates is significantly high and is predominantly mediated by the ermB aene. Isolates that carrv both ermB and mefA Qenes are oredominantlv of serotvoes 19A and 19E

关 键 词：Streptococcus pneumonia invasive pneumococcal diseases  erythromycin resistance  genes 

分 类 号：R378[医药卫生—病原生物学]