巴戟天根两种提取物对微波损伤大鼠生精功能的影响  

Morinda Officialis How extract improves microwave-induced reproductive impairment in male rats

作　　者：王凤娟[1] 王玮[1,2] 李容[3] 宋斌[1,2] 张永红[4] 周义湘 

机构地区：[1]福建医科大学神经生物学研究中心,福建福州350108 [2]福建医科大学人体解剖与组织胚胎学系,福建福州350108 [3]泉州医学高等专科学校人体解剖学教研室,福建泉州362001 [4]福建医科大学药学院,福建福州350108

出　　处：《中华男科学杂志》2013年第4期340-345,共6页National Journal of Andrology

基　　金：福建省重大科技项目(2012Y0032)~~

摘　　要：目的:探讨巴戟天根不同浓度提取物对微波损伤的雄性SD大鼠生精功能的影响。方法:40只健康雄性SD大鼠先分为正常对照组和辐射组,辐射后再将辐射组分为辐射模型组,巴戟天根水提物治疗组和巴戟天根醇提物治疗组,每组10只。辐射组应用微波信号发生器(900 HZ 1.0 W),功率密度为218μm/cm2,12 h/d,持续辐射2周。治疗组在辐射后分别给予巴戟天根水提物和巴戟天根醇提物20 g/(kg.d)持续灌胃2周。观察各组大鼠生长发育,扑捉潜伏期(CIP)和扑捉次数(CT),睾丸和附睾指数及精子形态学的差异,精子浓度、精子畸形率以及血清睾酮的浓度。结果:与正常对照组[(269.50±36.07)g]相比,辐射模型组[(254.77±20.38)g]大鼠体重稍降低,CIP延长及CT减少(P<0.05),精子浓度[(87.717±12.365)×106/ml]降低及精子畸形率[(0.126±0.100)×106/ml]明显升高(P<0.05);睾丸和附睾出现不同程度的病理损伤改变,睾丸指数降低,血清睾酮水平无明显变化。两治疗组较正常对照组体重显著下降(P<0.05),血清睾酮的水平显著升高,且与辐射模型组相比CT增加、CIP缩短、精子浓度显著升高、畸形率显著下降、血清睾酮的水平升高(P<0.05)。治疗组睾丸的病理性损伤显著修复,附睾管内除见大量精子外,还可见大量脱落的细胞。结论:巴戟天根的水提取物和醇提取物均可促进微波辐射损伤的生殖器官的修复以及精子的生成。Objective： To investigate the effects of different concentrations of Morinda Officialis How （MOH） extracts on mi- crowave radiation-induced injury to the spermatogenic function of male rats. Methods ： Forty SD male rats were equally divided into four groups： control, microwave injury model, aqueous extract of MOH treatment, and alcohol extract of MOH treatment. Models of microwave-induced injury were made by exposing the rats to microwave radiation from a microwave signal generator （900 MHz 1.0 W） at 218 μm/cm2, 12 h/d, for 2 weeks. The model rats of the two treatment groups were intragastrically given aqueous extract and alco- hol extract of MOH, respectively, both at 20 g per kg per day for 2 weeks. Then we observed the growth, capture incubation period （ CIP）, capture times （CT）, changes in testicular and epididymal weight and morphology, sperm concentration and malformation, and levels of serum testosterone. Results ： Compared with the controls, the rats of the model group showed a slightly reduced body weight, markedly prolonged CIP and decreased CT （ P 〈 0.05 ）, significantly reduced sperm concentration （ P 〈 0.05 ） and remarkably in-creased sperm malformation （P 〈 O. 05）, but no statistically significant differences in the testosterone level. The two treatment groups exhibited obviously decreased body weight, CIP and sperm malformation compared with the control group （ P 〈 O. 05 ） but markedly in- creased CT, sperm concentration and testosterone level as compared with the models （P 〈 O. 05 ）. The microwave radiation-induced testis injury was repaired perfectly in the two treatment groups, the epididymal ducts filled with sperm and cast-off cells. Conclusion ： Both aqueous and alcohol extracts of MOH can promote spermatogenesis and repair of reproductive injury induced by microwave radiation.

关 键 词：微波辐射 巴戟天 睾丸 附睾 血清睾酮 

分 类 号：R285.5[医药卫生—中药学]