桃蛀螟成虫Orco嗅觉受体基因的克隆及组织表达谱分析  

Cloning and tissue expression profiling of the olfactory receptor coreceptor gene in adults of Conogethes punctiferalis (Lepidoptera: Crambidae)

作　　者：葛星[1,2] 张天涛[2] 何康来[2] 王勤英[1] 李云龙 王振营[2] 

机构地区：[1]河北农业大学植物保护学院,河北保定071000 [2]中国农业科学院植物保护研究所、植物病虫害生物学国家重点实验室,北京100193 [3]北京市植物保护站,北京100032

出　　处：《昆虫学报》2013年第3期243-250,共8页Acta Entomologica Sinica

基　　金：现代农业产业技术体系建设专项(CARS-02);北京市科技计划项目(Z111100056811009)

摘　　要：【目的】克隆桃蛀螟Conogethes punctiferalis(Guenée)的Orco嗅觉受体基因,并研究其在不同组织的表达谱。【方法】利用PCR技术克隆桃蛀螟触角Orco基因,对该基因编码的氨基酸序列进行生物信息学分析,并利用荧光定量PCR技术分析该基因在的表达量。【结果】获得桃蛀螟成虫Orco的cDNA全长序列,并命名为CpunOrco(GenBank登录号:JX101681)。该基因的开放阅读框全长1425bp,编码475个氨基酸,序列中有7个跨膜区。对桃蛀螟成虫不同组织中CpunOrco的荧光定量PCR结果表明,CpunOrco主要在触角和下颚须中表达,雄虫触角中的表达量高于雌虫,并且该基因在其他组织中也有一定的表达。【结论】本研究明确了该嗅觉受体基因在桃蛀螟成虫不同组织内的表达水平,为进一步研究其功能提供了理论依据。[ Aim ] To clone the olfactory receptor co-receptor （Orco） gene in the yellow peach moth Congethes punctiferalis （Guenee） and analyze its expression level in different tissues. [ Methods ] The Orco gene was cloned by polymerase chain reaction （PCR）, and its expression level was analyzed by real-time quantitative PCR. [ Results ] The Orco gene was cloned from adults of C. punctiferalis, and named as CpunOrco （GenBank accession no. JX101681 ）. Sequence analysis revealed that the open reading frame of CpunOrco is 1 425 bp in length, encoding 475 amino acid residues, with seven transmembrane domains. Real-time quantitative PCR results indicated that CpunOrco was mainly expressed in the antennae and maxillary palpi of C. punctiferalis, while expressed in other tissues at a relatively low level. The expression level of CpunOrco was higher in antennae of male adults than in antennae of female adults. [ Conclusion ] This study makes clear the expression level of CpunOrco in different tissues of C. punctiferalis adults and provides a basis for further functional study of this gene.

关 键 词：桃蛀螟 嗅觉受体蛋白 基因克隆 荧光定量PCR 组织表达谱 

分 类 号：Q966[生物学—昆虫学]