七星瓢虫成虫石油醚提取物的化学成分及抑菌活性  

Chemical composition and antimicrobial activity of petroleum ether extracts from adults of Coccinella septempunctata (Coleoptera: Coccinellidae)

作　　者：孙涛[1,2] 张雅梅[2] 张霞[3] 田野[2] 李亚威[2] 刘文丛[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]吉林大学植物科学学院,长春130062 [3]白求恩医科大学药厂,长春130012

出　　处：《昆虫学报》2013年第3期257-262,共6页Acta Entomologica Sinica

基　　金：吉林省科技厅项目(20110904)

摘　　要：【目的】确定七星瓢虫Coccinella septempunctata成虫石油醚提取物的化学成分及其抑菌活性。【方法】用甲醇室温提取,石油醚、乙酸乙酯和正丁醇梯度萃取,利用气相色谱-质谱连用仪(Gas Chromatograph-Mass Spectrometer,GC-MS)对七星瓢虫成虫石油醚提取物成分进行分析,通过硅胶、凝胶Sephadex LH-20、ODS-C18柱层析法对正丁醇提取部分的成分进行分离,采用质谱和核磁共振等手段鉴定结构。采用纸片扩散法对七星瓢虫成虫石油醚提取物的抑菌活性进行测试。【结果】经GC-MS联用技术从石油醚相中鉴定出14个化合物,从正丁醇相中分离鉴定了7个化合物。石油醚提取物对肠道沙门氏菌Salmonella enterica有抑制作用,在浓度为100和50mg/mL时,抑菌率分别为52.78%和27.05%,在该浓度下对绿脓杆菌Pseudomonas aeruginosa、玉米大斑病菌Exserohilum turcicum和水稻纹枯病菌Rhizoctonia solani没有表现出明显的抑制作用。【结论】七星瓢虫C.septempunctata成虫石油醚提取物对肠道沙门氏菌具有抑制作用。[ Aim] To explore the chemical composition and the antimicrobial activity of petroleum ether extracts from Coccinella septempunctata. [ Methods] The petroleum ether extracts from C. septempunctata adults were identified by using gas chromatography-mass spectrometry （GC-MS）, the compounds of n-butanol ether extracts were isolated by Sephadex LH-20, ODS-Cls and silica gel column chromatographic methods, the structures of these compounds were identified on the basis of physicochemical and spectroscopic analysis, and the antimicrobial activity of the petroleum ether extracts against Salmonella enterica, Pseudomonas aeruginosa, Exserohilum turcicum and Rhizoctonia solani was measured by a disk agar diffusion method. [ Results ] Fourteen compounds were identified from the petroleum ether extracts by GC-MS. Seven compounds were isolated and identified from n-butanol ether extracts, which were allantoin, thymine, 4-hydroxy-phenylethyl alcohol, anthranilic acid, adenosine, succinic acid and mannitol. The petroleum ether extracts at the concentration of 100 and 50 mg/mL had an inhibitory effect against S. enterica, with the inhibition rates of 52.78% and 27.05%, respectively, but had no obvious activity against other tested microbes. [ Conclusion ] The results of this study prove that the petroleum ether extracts from C. septempunctata adults have inhibitory activities against S. enterica.

关 键 词：石油醚提取物 七星瓢虫 抗菌活性 化学成分 成人 邻氨基苯甲酸 交联葡聚糖凝胶 GC-MS 

分 类 号：TQ424.3[化学工程] S572[农业科学—烟草工业]