铃木氏果蝇不同地理种群中Wolbachia的检测和系统发育分析  

Detection and phylogenetic analysis of Wolbachia in different geographical populations of Drosophila suzukii (Diptera: Drosophilidae)

作　　者：于毅[1] 王静[1] 陶云荔[2] 国栋[2] 褚栋[2] 

机构地区：[1]山东省农业科学院植物保护研究所、山东省植物病毒学重点实验室,济南250100 [2]青岛农业大学农学与植物保护学院、山东省植物病虫害综合防控重点实验室,山东青岛266109

出　　处：《昆虫学报》2013年第3期323-328,共6页Acta Entomologica Sinica

基　　金：山东省现代农业产业技术体系水果团队;农业部农业科研杰出人才项目;“泰山学者”建设工程专项经费资助

摘　　要：铃木氏果蝇Drosophila suzukii是原产于东南亚地区的重要果树害虫,近年来传入北美和欧洲等地区造成严重的危害。本研究利用Wolbachia的16S rDNA和wsp基因特异引物(分别为16S-F/16S-R和81F/691R)对铃木氏果蝇7个地理种群(中国的5个种群、韩国的1个种群和美国的1个种群)的Wolbachia进行了PCR检测并对检测结果进行了比较;对感染个体体内Wolbachia的16S rDNA基因片段进行测序,确定了我国铃木氏果蝇体内Wolbachia的分类地位。基于Wolbachia的16S rDNA基因特异引物检测结果发现,我国5个铃木氏果蝇种群广泛感染Wolbachia(感染率36.7%～80.0%),而韩国和美国2个种群均未检测到该菌的感染。而利用wsp基因特异引物无法检测到该菌。基于Wolbachia的16S rDNA基因构建系统发育树表明,我国铃木氏果蝇种群感染的Wolbachia全部属于A组。这些结果为研究Wolbachia感染对铃木氏果蝇生物学及生态学的影响奠定了基础。Drosophila suzukii is an important fruit pest native to Southeast Asia. During the past several years, it has been introduced in North America and Europe where it has caused severe damages. In this study, the infection of Wolbachia in seven geographical populations of D. suzukii （five Chinese populations, one Korean population, and one American population） was detected using the 16S rDNA and wsp specific primers （16S-F/16S-R and 81F/691R, respectively）, the infection charateristics of Wolbachia within the seven populations were compared, and the 16S rDNA gene fragements within the populaitons were sequenced and then used to determine the classification of Wolbachia. Based on the 16S rDNA primers, we found that all five Chinese populations were infected with Wolbachia with a high infection rate （ ranged from 36.7% to 80.0% ）, while the population from Korea and the United States was not infected with this symbiont. However, the infection of Wolbachia was not detectable using the wsp specific primers. The phylogenetic tree constructed with 16S rDNA gene shows that all of Wolbachia within Chinese populations belong to group A. The results lay a foundation for revealing the biological and ecological effects of Wolbachia on the host D. suzukii.

关 键 词：铃木氏果蝇 地理种群 内共生菌 WOLBACHIA 16S rDNA WSP 系统发育分析 

分 类 号：Q965.8[生物学—昆虫学]