重庆地区5岁以内儿童腹泻病毒病原及流行病学特点  

Etiological and epidemiological characters of diarrhea virus in children at ages of less than 5 years in Chongqing Region,China

作　　者：任增志[1,2] 孔元梅[1,2] 王倩倩[1,2] 黄爱龙[3] 钟晓妮[4] 许红梅[2] 

机构地区：[1]儿童发育疾病研究教育部重点实验室儿科学重庆市重点实验室重庆市儿童发育重大疾病诊治与预防国际科技合作基地,重庆400014 [2]重庆医科大学附属儿童医院感染消化科,重庆400014 [3]重庆医科大学感染性疾病分子生物学教育部重点实验室,重庆400014 [4]重庆医科大学公共卫生学院卫生统计学教研室,重庆400014

出　　处：《中国生物制品学杂志》2013年第4期548-552,共5页Chinese Journal of Biologicals

基　　金：传染病重大专项传染病监测平台项目(2009zx10004-212)

摘　　要：目的了解重庆地区5岁以内儿童腹泻病毒病原及流行病学特点。方法收集重庆医科大学附属儿童医院2010年8～11月就诊的5岁以内腹泻患儿的粪便标本共500份,采用胶体金法检测A组轮状病毒(Rotavirus,RV),RT-PCR法检测B、C组RV、诺如病毒(Norovirus,NV)GⅠ和GⅡ、肠道腺病毒(Adenovirus,ADV)、札如病毒(Sapovirus,SLV)和星状病毒(Astrovirus,ASV),取NV和SLV阳性PCR产物测序,并对病毒基因进行分型。采用MEGA 5.05软件构建进化树,Kimura’s two-parameter法计算遗传距离,邻接法(Neighbor-joining)boot-strap重复检验1 000次。结果 500份标本中,检测到A组RV阳性标本134份,阳性率为26.8%;NV GⅡ型阳性标本132份,阳性率为26.4%;ADV阳性标本31份,阳性率为6.2%;SLV阳性标本9份,阳性率为1.8%;ASV阳性标本1份,阳性率为0.2%;未检测到B、C组RV和NV GⅠ型。随机选择22份NV阳性标本进行测序及基因分型,其中GⅡ/4占绝对优势,其次为GⅡ/6、GⅡ/2、GⅡ/3和GⅡ/7。SLV可分为4个基因亚型,其中GⅠ/1为优势株,其次为GⅠ/2、GⅡ/1和GⅠV 1。结论重庆地区5岁以下儿童腹泻以病毒感染为主,RV是主要的病原体,其次为NV、ADV、SLV和ASV。Objective To investigate the etiological and epidemiological characters of diarrhea viruses in children at ages of less than 5 years in Chongqing Region,China.Methods A total of 500 fecal samples were collected from children,at ages of less than 5 years,who visited Children＇s Hospital of Chongqing Medical University from August to November 2010,and tested for rotavirus（RV） group A by colloidal gold method,while for RV groups B and C,norovirus（NV） GⅠ and GⅡ,enteric adenovirus（ADV）,sapovirus（SLV） and astrovirus（ASV） by RT-PCR.The PCR products positive for NV and SLV were sequenced,of which the genotypes were then classified using the clustering methods reported previously.The genetic distances were calculated by Kimura＇s two-parameter method.Phylogenetic trees with bootstrap analysis from 1 000 replicas were constructed using the MEGA program version 5.05 by the neighbor-joining method.Results RV group A was the most frequent virus identified in 134 of 500 samples（26.8%）,followed by NV GⅡ（132 / 500,26.4%）,ADV（31 / 500,6.2%）,SLV（9 / 500,1.8%） ASV（1 / 500,0.2%）.However,no RV groups B and C or NV GⅠ were detected.Twenty-two NV-positive samples were selected randomly for sequencing and genotyping,in which GⅡ/ 4 was defined as the predominant strain,followed by GⅡ / 6,GⅡ / 2,GⅡ / 3 and GⅡ / 7.SLV was classified into 4 genotypes,of which GⅠ/ 1 was predominant,followed by GⅠ/ 2,GⅡ/ 1 and GⅠV 1.Conclusion Enteric viruses are a major cause of diarrhea in children at ages of less than 5 years in Chongqing Region,of which RV is the most common etiological agent,follow by NV,ADV,SLV and ASV.

关 键 词：腹泻 婴幼儿 肠道病毒 进化分析 

分 类 号：R373.2[医药卫生—病原生物学] Q755[医药卫生—基础医学]