油包水型芍药苷微乳在大鼠肠道的吸收动力学研究  

Studies on absorption kinetics of paeoniflorin microemulsion (W/O) in intestine of rat

作　　者：袁君[1] 王春[1] 杨昭毅[1] 聂欣欣[1] 魏伟[1] 

机构地区：[1]安徽医科大学临床药理研究所,抗炎免疫药理学教育部重点实验室,合肥230032

出　　处：《安徽医科大学学报》2013年第5期481-484,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30973543;81173075)

摘　　要：目的比较油包水型芍药苷微乳[PaeME(W/O)]和芍药苷(Pae)水溶液在大鼠肠道的吸收情况。方法采用大鼠在体肠回流模型,通过高效液相色谱(HPLC)法分别测量肠循环液中的PaeME(W/O)与Pae的含量,计算出PaeME(W/O)与Pae在大鼠肠道的吸收药量、吸收速率常数值(Ka)和吸收半衰期(t1/2)。结果在2～20μg/ml浓度范围内PaeME(W/O)的吸收量与浓度呈线性关系,PaeME(W/O)的Ka值基本不变;PaeME(W/O)在十二指肠、回肠和结肠吸收药量均明显高于Pae;PaeME(W/O)型制剂在十二指肠、回肠和结肠的t1/2均低于相应的肠段的Pae。结论 PaeME(W/O)在整个肠段均有吸收,PaeME(W/O)在肠道吸收要明显好于Pae。Objective To compare the absorption kinetics of paeoniflorin microemulsion（PaeME） and Pae solution in rat＇ s intestine. Methods Intestinal in situ reflux models for rats were used. The concentration of PaeME and Pae in intestine flux was measured by HPLC, which was used to calculate absorptive amounts, absorptive rate （Ka） and absorptive half-life （ t1/2 ）. Results When the concentration was raised from 2. 0 to 20. 0 mg/L, the uptake of paeoniflorin was increased linearly, and the absorption rate kept at the same level. Moreover, the uptake of PaeME in duodenum, ileum, and colon was more aboundant than that of Pae. By contrast, t1/2 of PaeME in these sections was lower than that of Pae. Conclusion PaeME is absorbed in the whole intestine, and the uptake of PaeME is more than that of Pae solution.

关 键 词：芍药苷 微乳 高效液相色谱法 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]