多氯联苯118对大鼠甲状腺自身免疫反应的影响  

Effects of polychlorinated biphenyl 118 on thyroid autoimmunity in rats

作　　者：汤金梅 李文 解雨春 郭红伟 程培 陈欢欢 郑旭琴 蒋琳 丁国宪 刘云 段宇 

机构地区：[1]南京医科大学第一附属医院内分泌科,江苏省210029

出　　处：《江苏医药》2013年第8期869-871,F0002,共4页Jiangsu Medical Journal

基　　金：国家自然科学基金(81170726);江苏省医学重点人才(20111541);南京医科大学第一附属医院创新团队(20113012);江苏高校优势学科建设工程(PAPD)

摘　　要：目的探讨多氯联苯118(PCB118)对大鼠甲状腺功能及自身免疫反应的影响。方法Wistar大鼠40只均分为四组:A组为空白对照;B、C、D组分别给予PCB118 10、100、1000μg.kg-1.d-1腹腔注射,每周5次。13周后检测血清游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、促甲状腺激素(TSH)、甲状腺球蛋白(TG)、甲状腺球蛋白抗体(TGAb)、过氧化物酶抗体(TPOAb)水平,甲状腺组织过氧化物酶(TPO)mRNA表达水平,观察甲状腺组织病理学改变。结果甲状腺组织病理学检查显示B、C、D组均可见滤泡腔塌陷、上皮脱落、间质纤维化、小血管增生,B、C组可见间质淋巴细胞浸润。随着PCB118剂量增加,B、C、D组血清FT3、FT4、TG浓度逐渐降低;D组FT3浓度明显低于A、B组(P<0.05);B、C、D组血清FT4、TSH浓度明显低于A组(P<0.05);C、D组血清TG水平明显低于A组(P<0.05)。与A组比较,B、C组血清TGAb、TPOAb浓度显著升高(P<0.05);C组TPO mRNA表达水平明显高于A组(P<0.05)。结论 PCB118可导致大鼠甲状腺组织形态和功能异常,激活甲状腺自身免疫反应。Objective To study the effects of polychlorinated biphenyl ll8（PCBll8） on thyroid function and autoimmunity in rats. Methods Fourty Wistar rats were equally assigned into 4 groups. The rats in group A were taken as blank controls. The rats in groups of B,C and D were treated with intraperitoneal injection of PCB118 10,100 and 1000 μg . kg-1 . d-1 , 5 times a week for 13 weeks. The histopathology of the thyroid was observed under light microscopy. Serum levels of free thyroxine （ FT4 ）, free triiodothyronine （ FT3 ）, thyroid-stimulating hormone （ TSH ）, thyroglobulin （TG）, thyroglobulin antibody （TGAb）, and thyroid peroxidase antibody （TPOAb） were measured by radioimmunoassay. The mRNA expression of thyroperoxidase（TPO） in thyroid tissues was quantified by RT-PCIL Results The distinct histopathological changes of the thyroids, such as collapsed follicles,mesenchyme fibrosis and vascularization were seen in groups of B, C and D. Interstitial lymphocytic infiltration was observed in groups of B and C as well. Along with increasing doses of PCBll8,serum levels of FT3, FT4 and TG were decreased, in which serum level of FT3 was significantly lower in group D than that in groups of A and B（P〈0. 05）. Serum levels of FT4 and TSH were lower in groups of B,C and D than those in group A（P〈0. 05）. Serum level of TG was lower in groups of C and D than that in group A（P〈0. 05）. Serum levels of TGAb and TPOAb were higher in groups of B and C than thoae in group A（P〈0. 05）. The TPO mRNA expression was higher in group C than that in group A（P〈0. 05）. Conclusion PCBll8 could cause abnormal changes of thyroid in morphological structure and function,activate the autoimmunity of the thyroid.

关 键 词：多氯联苯118 甲状腺功能 

分 类 号：R581[医药卫生—内分泌]