拟南芥抗旱突变体vem1对NaCl和ABA胁迫的响应  

Responses of the Drought-Resistent vem1 Mutant to NaCl and ABA Stress in Arabidopsis

作　　者：杨杰[1] 江力[2] 张娇娇[2] 陈子平[2] 

机构地区：[1]合肥师范学院生命科学系,合肥230601 [2]合肥工业大学生物与食品工程学院,合肥230009

出　　处：《植物生理学报》2013年第4期337-342,共6页Plant Physiology Journal

基　　金：安徽省级自然科学研究重大项目(KJ2011ZD08);安徽省自然科学基金项目(1208085MC47)

摘　　要：我们从拟南芥突变体库中筛选获得一抗旱突变体vem1。本文通过观察突变体表型,测定其脯氨酸、可溶糖、叶绿素含量和相对电导率等抗逆相关的生理指标,研究突变体vem1对NaCl和ABA胁迫的响应。NaCl胁迫下,突变体vem1比野生型(WT)积累更多的脯氨酸和可溶性糖,而相对电导率、叶绿素含量均比WT低。ABA胁迫下,vem1积累的脯氨酸、可溶糖和叶绿素含量均低于WT,而相对电导率和胁迫相关基因RD29A表达量高于WT。结果表明,vem1对NaCl胁迫具有一定的抗性,对ABA胁迫表现敏感。研究为抗旱基因VEM1克隆及功能分析奠定基础。We have isolated a drought-resistent mutant veml from the Arabidopsis mutant pool. In this work, wild-type （WT） and veml phenotypes were observed, and resistance-related physiological indexes, including proline content, soluble carbohydrate, chlorophyll content, and relative conductivity were measured to study re- sponse of mutant veml to NaC1 and ABA stress. Under NaC1 stress, veml mutant plants showed higher proline and soluble sugar levels but lower relative conductivity and chlorophyll content compared with WT plants. Un- der ABA stress, contents of proline and soluble sugar as well as chlorophyll in veml mutant plants were lower than those of WT plants; however, relative conductivity and the expression level of related stress-responsive gene RD29A in the veto1 mutant were higher than those in WT. These results suggest that the veto1 mutant was more resistant to NaC1 stress but sensitive to ABA stress. This study provides a basis for cloning and functional analysis of the drought-resistant gene VEM1.

关 键 词：拟南芥 抗旱突变体vem1 甘露醇 NACL胁迫 ABA胁迫 

分 类 号：Q945.78[生物学—植物学]