大鼠腹主动脉瘤形成过程中T细胞及CD4＋CD28-T细胞的动态变化及意义  

T cell and CD4 ＋ CD28- T cell changes in the occurrence of rat abdominal aortic aneurysm

作　　者：许文平[1] 谢志泉[2] 李志樑[3] 邱健[2] 吴自强[2] 傅锐斌[2] 

机构地区：[1]广州军区广州总医院OICU,广州510010 [2]广州军区广州总医院心血管内科,广州510010 [3]广州南方医科大学珠江医院心内科

出　　处：《中华普通外科杂志》2013年第4期296-299,共4页Chinese Journal of General Surgery

基　　金：广东省医科学基金资助项目（A2005505）

摘　　要：目的探讨T淋巴细胞及其亚群在腹主动脉瘤发病中的作用。方法50只Wistar大鼠随机分成A、B、C、D、E5组，每组10只，A组为正常对照组，B、C、D、E组分别为术后3、7、14、28d组，运用猪胰弹力酶灌注法建立腹主动脉瘤模型。免疫组化方法检测上述各组大鼠腹主动脉局部T、B淋巴细胞及巨噬细胞的表达水平，流式细胞术检测各组大鼠外周血中CD4＋ CD28-T淋巴细胞亚群的分布变化。结果大鼠腹主动脉局部T、B淋巴细胞及巨噬细胞表达的变化：术后7dT细胞显著升高（404-15，P〈0．05），B细胞及巨噬细胞有升高，但差异无统计学意义（P〉0．05）；术后14d，3种细胞表达升高最为显著（P〈0．05）；术后28d，3种细胞的表达较术后14d明显下降。术后各组大鼠外周血CD4＋、CD28-T细胞均有升高（P〈0．05），以术后7d比例最高（8．1％±2．1％，P〈0．05）。结论T淋巴细胞是腹主动脉瘤形成过程中最重要的炎症细胞之一，T淋巴细胞的浸润及其亚群分布啻化所致的角痦及餐痒反应在腹主动脉瘤形成早期可能趁着雷兽的触发及调节作用．Objective To evaluate T cell and CD4 ＋ CD28-T in the development and progression of abdominal aortic aneurysms （AAAs）. Methods Fifty Wistar rats were randomly divided into 5 groups （n = 10 each ）. An AAA animal model is established by enhancing perfusing elastase to the infrarenal abdominal aorta of the rats. The levels of T cell, B cell and maerophage cell of abdominal aorta of the rats on days 3,7, 14 and 28, were detected by enzyme linked immunosorbent assay（ELISA）. CD4 ＋ CD28-T cell of the peripheral blood were measured by flow cytometry. Result There was significant T-lymphocyte infiltration both in middle and outer membrane of the artery of the rats on day 7 after surgery. T-lymphocyte, B-lymphocyte and maerophage cell infiltration were on the peak in middle and outer membrane of the artery on day 14 after surgery. The ratio of CIM ＋ CD28- T cell in rat peripheral blood reached peak on day 7 （P 〈 0. 05）. Conclusions T cell and CD4＋ CD28- T cell expression increased in peripheral blood and abdominal aorta in AAA rat model, suggesting a potential role of T cell and CD4 ＋ CD28- T cells in the pathogenesis of AAAs, especially during the early develooment of AAAs.

关 键 词：主动脉瘤 腹 T淋巴细胞 炎症 免疫 

分 类 号：R54[医药卫生—心血管疾病]