不同鸭种胚胎期和岀雏早期生长轴GH、GHR和IGF-Ⅰ基因mRNA差异表达分析  

The Expression Analysis of Growth Axis Genes (GH, GHR and IGF-Ⅰ) during Embryonic and Post-Hatch Development in Different Duck Breeds

作　　者：单艳菊[1] 束婧婷[1] 徐文娟[1] 章双杰[1] 宋卫涛[1] 胡艳[1] 李慧芳[1] 

机构地区：[1]江苏省家禽科学研究所,家禽遗传育种重点实验室,扬州225125

出　　处：《农业生物技术学报》2013年第4期421-427,共7页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.31172194);江苏省科技支撑计划项目(No.BE2011329);现代农业品种创新项目(No.CX(11)1030);扬州市市科技攻关(No.YZ2010048)

摘　　要：为探讨生长轴生长激素(GH)、生长激素受体(GHR)和胰岛素样生长因子1(IGF-Ⅰ)基因在鸭早期生长发育中的作用,本研究采用实时荧光定量PCR方法研究生长速度不同的高邮鸭和金定鸭(Anas platyrhynchos Domestica)13、17、21、25、27胚龄和出雏后7日龄时垂体GH基因与胸肌和腿肌GHR和IGF-Ⅰ mRNA的表达水平。结果表明,这3个基因在两个鸭种中均有表达,且同一基因在不同鸭种的同一组织中有相似的表达规律。两个鸭种垂体GH mRNA表达水平均随着胚龄(或日龄)的增加而增加,且在7日龄时高邮鸭极显著高于金定鸭(P<0.01)。GHR mRNA在胸肌和腿肌中的表达均不存在品种差异。两个鸭种胸肌IGF-Ⅰ mRNA表达量,除27胚龄时高邮鸭略低于金定鸭外,其它各胚龄均是高邮鸭高于金定鸭,且在13、21胚龄和7日龄时呈显著或极显著差异(P<0.05或P<0.01);两个鸭种腿肌IGF-Ⅰ mRNA表达量均是高邮鸭高于金定鸭,但不存在显著的品种效应(P>0.05)。研究结果提示,这3个基因在鸭早期生长发育中具有重要作用,mRNA的表达具有明显时空性,GH和IGF-Ⅰ mRNA表达还存在品种差异性。This experiment was designed to analyse the role of growth axis genes including growth hormore （GH）, growth hormore receptor（GHR） and insulin-like growth factor I（IGF- I ） during embryonic and post- hatch development in two duck breeds（Gaoyou ducks and Jinding ducks）（Anas platyrhynchos Domestica） with different growth rate. The expression level of GH gene in pituitary tissue and GHR and IGF- I gene in pectorale muscle and leg muscle were quantified by Real time-PCR on days 13, 17, 21, 25 and 27 of embryonic development, as well as at 7 day post-hatching. The results showed that the mRNA of all the three genes could be detected in the studied tissues in both duck breeds, and relatively similar expression profile was found in the same tissue for each gene. The expression level of GH gene in pituitary increased throughout the embryonic and post-hatch development, and the expression level in Gaoyou ducks was extremely significantly higher than that in Jinding ducks at 7 day post-hatching （P〈0.01）. In pectoral muscle and leg muscle, the differences of the GHR gene expression between the two breeds at the same development stage were not significant. Within the breed, the expression level of GHR gene in pectoral muscle was significantly lower than that in leg muscle on 13 and 21 embrynic development（P〈0.05 or P〈0.01）, while the expression level of GHR gene in pectoral muscle was significantly higher than that in leg muscle at 25 and 27 embryonic age（P〈0.05 or P〈0.01）. The expression level of IGF- I gene in pectoral muscle in Gaoyou ducks was higher than that in Jinding ducks except for 27 embryonic age, and the differences were significant on 13, 21 embryonic development and 7 day post-hatch（P〈0.05 or P〈0.01）. The expression level of IGF- I gene in leg muscle of Gaoyou ducks was higher than that of Jinding ducks at different development stage, but the difference was not significant（P〉0.05）. Within the same breed, the expression level of IGF- I gene in pectoral musc

关 键 词：鸭 胚胎期 GH GHR IGF-Ⅰ 实时荧光定量PCR 

分 类 号：S852.4[农业科学—基础兽医学]