新型γ-聚谷氨酸自组装纳米胶束的制备及用于蛋白载体的研究  

Preparation of a Novel Self-assembly Nanoparticle Based on Amphiphilic γ-Polyglutamic Acid Derivatives as a Protein Carrier

作　　者：陈宽婷[1] 姚俊[1] 阮文辉 魏钦俊[1] 鲁雅洁[1] 曹新[1] 

机构地区：[1]南京医科大学基础医学院,南京2100292 [2]山西省医药与生命科学研究院,太原030001

出　　处：《中国生物工程杂志》2013年第4期101-105,共5页China Biotechnology

基　　金：山西省科技攻关项目(20110321081-01;20120313016-6);江苏高校优势学科建设工程资助项目

摘　　要：对水溶性的γ-聚谷氨酸(γ-PGA)进行了接枝改性,合成了两亲性γ-聚谷氨酸(γ-PGA)接枝衍生物,采用超声探头法制备胆甾醇基γ-PGA自组装胶束,并以卵清蛋白(OVA)作为模型蛋白,研究其载药和释药性能。结果表明,制备的两亲性胆甾醇基γ-PGA自组装胶束平均粒径为299.6+27.3nm,粒径的多分散系数较窄(0.17),且具有较低的细胞毒性;其疏水核-亲水壳的纳米微结构对蛋白药物显示了良好载药性能,对OVA载药量可达118.8μg/mg,包封率33.5%;体外释药结果显示,负载OVA的甾醇基γ-PGA自组装胶束能延缓蛋白的释放,释药速率与介质pH密切相关。A novel amphiphilic graft copolymer composed of hydrophilic backbone and cholesterol as the hydrophobic segment was ,γ-polyglutamic acid （γ-PGA） as synthesized. The cholesterol-bearing the PGA （γ-PGA-Graft-CH） was used to form self-assembly nanoparticles （γ-PGA-Graft-CH NPs） with an inner hydrophobic core and an outer hydrophilic shell via the ultrasonic probe method. The obtained nanoparticles showed low cytotoxicity and a narrow size distribution （ PDI = 0. 17 ） with a mean diameter 299.6 ＋ 5.4 nm. OVA-loading -γ-PGA-Graft-CH NPs was also successfully prepared, with drug loading content of 118.8μg/mg, and entrapment efficiency of 33.5%. The experimental results also showed that OVA continuously released from γ-PGA-Graft-CH NPs in the phosphate buffered saline （PBS） solutions, and its release was sensitive to the pH of the release medium.

关 键 词：Γ-聚谷氨酸 接枝共聚物 自组装纳米胶束 药物载体 

分 类 号：Q819[生物学—生物工程]