弗氏柠檬酸杆菌临床分离株整合子与耐药相关性的研究  

Correlation between int, egron and drug-resistance in clinical isolates of Citrobacter freundii

作　　者：俞燕[1] 魏取好[1] 王卫忠[2] 

机构地区：[1]浙江省人民医院手外科,杭州310014 [2]浙江省人民医院检验医学中心,杭州310014

出　　处：《国际流行病学传染病学杂志》2013年第2期106-109,共4页International Journal of Epidemiology and Infectious Disease

基　　金：浙江省医学重点学科基金（07-010）;浙江省中医药科研基金（2012ZA017）

摘　　要：目的了解临床分离弗氏柠檬酸杆菌中整合子的分布，分析整合子与细菌耐药性的关系。方法选取2011年7月至2013年2月非重复分离自浙江省人民医院就诊患者临床样本中的37株弗氏柠檬酸杆菌，用聚合酶链反应（PER）检测菌株第1、Ⅱ、Ⅲ类整合子。抗生素药敏试验采用微量肉汤稀释法。结果37株弗氏柠檬酸杆菌中，有14株（37．8％）检测到第1类整合子，未检出第Ⅱ类和第Ⅲ类整合子。对15种常用抗生素的耐药性检测显示，第1类整合子阳性菌株对复方磺胺甲曙唑及环丙沙星的耐药率高于第1类整合子阴性菌株（P均〈0．05）；对其他抗生素的耐药性，第1类整合子阳性和阴性菌株间差异无统计学意义（P均〉O．05）。结论第工类整合子在弗氏柠檬酸杆菌中分布普遍。第1类整合子与复方磺胺甲曙唑及环丙沙星的耐药性密切相关。Objective To investigate the distribution of integrons in clinical Citrobacter freundii st=fins and ana- lyze the relationship between integrons and antibiotic resistance.Methods A total of 37 non-repeated clinical isolates of Citrobacter freund//from Zhejiang Provincial People＇ s Hospital were selected from July 2011 to February 2013. The types of the interons（ intll, intI2, intl3） were identified by PCR and the drug-sensitivity tests were carried out by broth dilution method. Retmlts Among 37 non-repeated isolates of Citrobacter freundii, intI1 was detected in 14 isolates（37.8%）, intl2 and intI3 genes were not detected. After drug-resistance test of 15 common used antibiotics, the intll-positive strains showed higher resistant rates of trimethoprim-sulfamethoxazole and ciprofloxacin than the intll-negative strains （ P all 〈 0.05）. For resistance rates of the other antibiotics, there was no difference between the intll-positive and intll- negative strains（ P all 〉 0.05）. Conclusions In this study, the class I integron is extensively found in clinical isolates of Citrobater freundii. Integrons play an important role in drug resistance of trimethoprim-sulfamethoxazole and ciprofloxacin.

关 键 词：整合子 整合酶 耐药性 

分 类 号：R446.5[医药卫生—诊断学]