超声辐照联合微泡增强介导SPIO标记ADSCs及细胞内铁蛋白表达  

Enhancement of Ultrasonic Exposure Associated with Microbubble Mediation to ADSCs Labeling by SPIO and Its Effect on Intracellular Ferritin Expression

作　　者：王瑛[1] 叶素敏[1] 陈绮璐[1] 张晓云[1] 黄帼英[1] 刘再毅[2] 

机构地区：[1]广州医学院第一附属医院超声科,广东广州510120 [2]广东省人民医院广东省医学科学院放射科,广东广州510080

出　　处：《贵阳医学院学报》2013年第2期141-144,151,共5页Journal of Guiyang Medical College

基　　金：广东省医学科学技术研究基金(A2011036);广州医学院青年基金(2010A07)

摘　　要：目的:探讨超声辐照联合微泡增强介导超顺磁氧化铁(SPIO)标记脂肪干细胞(ADSCs),及其对干细胞内铁蛋白表达的影响。方法:使用超声辐照干细胞+超声微泡+SPIO细胞悬液标记ADSCs,使用普鲁士蓝染色检测细胞的SPIO标记情况,台盼兰染色检测细胞活力,并使用Western-blot对ADSCs内的铁蛋白轻链(Fn-L)表达进行检测。结果:普鲁士蓝染色可见ADSCs铁染色呈阳性,在胞浆内可见蓝色颗粒;台盼兰染色检测细胞活力实验组与对照组差异无统计学意义(P>0.05),标记后的ADSCs内的Fn-L表达在标记后2 h即升高。结论:超声辐照联合微泡增强可以有效对ADSCs进行磁探针标记,标记后细胞内铁蛋白升高。Objective： To investigate the feasibility of labeling adipose-derived stem cells （ADSCs） with superparamagnetic iron oxide （SPIO） mediated by ultrasonic exposure associated with microbubbles and its effect on intracellular ferritin light chain （Fn-L） expression. Methods. ADSCs were labeled with SPIO particles mediated by ultrasonic exposure and microbubbles. The labeling effect was evaluated by prussian blue staining, and viability of the labeled cells was assessed by trypan blue staining. Intracellular Fn-L level was examined by western blot. Results： Prussian blue staining demonstrated that iron staining was positive in ADSCs and blue particles located in the cytoplasm of the ADSCs. Trypan blue test showed that there was no statistical difference （ P 〉 0.05 ） in viability between la- beled and unlabeled groups . The intracellular Fn-L level increased in 2 hours after labeling. Conclusions： Ultrasound exposure combined with microbubble enhancement is effective in stem cell SPIO labeling, and in maintaining intracellular iron homeostasis by increasing intracellular Fn-L expression.

关 键 词：干细胞 超顺磁氧化铁 超声微泡 铁蛋白 

分 类 号：R445.2[医药卫生—影像医学与核医学]