成年克隆巴马小型猪心脏差异蛋白的鉴定与生物信息学分析  

Identification and Bioinformatic Analysis of Differentially Expressed Proteins of Hearts from Adult Cloned Bama Miniature Pigs

作　　者：张树山[1,2] 戴建军[1,2] 吴彩凤[1,2] 张廷宇[1,2] 张德福[1,2] 

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海201106 [2]上海市农业遗传育种重点实验室动物遗传工程研究室,上海201106

出　　处：《畜牧兽医学报》2013年第4期508-513,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家转基因生物新品种培育科技重大专项(2011ZX08006-005);上海市科委成果转化项目(123919N0700);上海市农业科学院青年基金(2011-07)

摘　　要：本研究旨在探索体细胞核移植(克隆)操作对克隆巴马小型猪心脏发育的影响。分别提取3头成年克隆猪和3头繁殖猪的心脏蛋白,利用双向电泳-质谱体系的比较蛋白质组学技术进行分离和鉴定,并结合生物信息学手段对部分差异蛋白进行功能分析。结果表明,与对照猪相比,克隆巴马小型猪的心脏蛋白共有11个差异蛋白,其中5个为上调蛋白,6个为下调蛋白。本试验在蛋白质水平上证实:3头克隆巴马小型猪的心脏均存在一定程度的功能失调。The aim of this study was to explore the influence of somatic cell nuclear transfer （namely clone） operation on cardiac development of cloned Bama Miniature pigs. The proteins from three adult cloned pigs and three normal reproductive pigs were extracted, separated and identified by using comparative proteomics containing two-dimensinal gel electrophoresis （2-DE） and mass spectrometry （MS）. And the functions of some important differential proteins was ana- lyzed by bioinformatics analysis. Compared with controls, there are 11 differential proteins inclu- ding 5 up-regulated and 6 down-regulated proteins in the hearts from adult cloned Bama Miniature pigs. In the present study, endothelial dysfunction of hearts from three adult cloned Bama Miniature pigs was confirmed at proteomics level.

关 键 词：巴马小型猪 体细胞核移植 心脏 比较蛋白质组学 

分 类 号：S828[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]