兔骨髓间充质干细胞向胰岛细胞分化的研究  

Study of Inducing Rabbit BMSCs into Islet Cells

作　　者：何俊丹[1,2] 张志帅 王新庄[1] 吕婧玉[1] 许晓婷[1] 蒋金航[1] 翟明胜[1] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]开封市动物疫病预防控制中心,开封475004 [3]河南省动物卫生监督所,郑州450008

出　　处：《畜牧兽医学报》2013年第4期543-548,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：河南省基础与前沿技术研究计划(092300410081);河南农业大学人才引进基金

摘　　要：旨在探讨体外诱导兔骨髓间充质干细胞向胰岛细胞分化的可行性,本研究采用二甲基亚砜联合高浓度葡萄糖对分离纯化的第3代兔BMSCs进行体外诱导,观察诱导前后细胞形态学变化,用双硫腙染色法鉴定胰岛细胞,用免疫细胞化学法检测胰岛素的表达,用ELISA法检测分泌到培养基中的胰岛素含量。结果,试验组在诱导7d出现细胞团样集落,10d获得排列紧密的细胞团,而对照组始终未见细胞团;经双硫腙染色试验组、免疫细胞化学染色试验组均显示阳性,对照组为阴性,ELISA检测试验组7和10d的胰岛素含量分别为(27.82±0.43)和(27.89±0.25)mU.L-1,与对照组的(23.39±0.20)和(25.71±0.27)mU.L-1比较,差异极显著(P<0.01)。结果表明,二甲基亚砜联合高糖能够在较短的周期内诱导出胰岛细胞。To explore the method for inducing rabbit bone marrow mesenchymal stem cells（BMSCs） into islet cells in vitro, BMSCs at 3rd generation were induced with dimethyl sulfoxide （DMSO） and high concentration of glucose. The morphological change of cells were observed after inducing. The islet cells were identified by Dithizone staining, the expression of insulin was detected by immunocytochemical staining. The insulin content was detected by ELISA. After induced 7 days,the cells appearred groups of cell mass. After 10 days, groups of cell mass got closely, whereas the control cells did not appeare group of cell mass. After treated with Dithizone （DTZ） and Immunocellulerchemistry （ICC）, the induced cells both were positive and the control group both were negative. In ELISA tests, the insulin content of induced group were （27.82±0.43） and （27.89±0.25） mU·L-1 at 7 and 10 days, that of the control group were （23.39±0.20） and （25.71±0.27）mU·L-1. The difference was significant（P〈0.01） between induced and control groups. The result showed that DMSO with high concentration of glucose can induce rabbit BMSCs into islet cells in a relatively short period.

关 键 词：兔 BMSCS 分化 二甲基亚砜 胰岛素 

分 类 号：S829.1[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]