红景天多糖对猪卵母细胞体外成熟及其发育潜力的影响  

Effect of Rhodiola Sachanensis Polysaccharide ( RSP ) on Developmental Competence of Porcine Oocytes Following Maturation and Culture in vitro

作　　者：徐利[1,2,3] 戴建军[1,2] 张廷宇[1,2] 吴彩凤[1,2] 张树山[1,2] 张德福[1,2] 顾晓龙[1,2] 李翔[1,2] 韩晓[1,2,3] 李芳芳[1,2,3] 

机构地区：[1]上海农业科学院畜牧兽医研究所,上海201106 [2]上海农业遗传育种重点实验室动物遗传工程研究室,上海201106 [3]上海海洋大学水产与生命学院,上海201306

出　　处：《畜牧兽医学报》2013年第4期557-561,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：上海市科委农业科技成果转化项目(103919N1800;123919NO700);上海市科技兴农推广项目(2007-3-7);上海市农科院青年科技发展基金(2011-07);国家转基因生物新品种培育科技重大专项(2008ZX08006-005;2009ZX08006-014B)

摘　　要：旨在探讨红景天多糖(Rhodiola sachalinensis polysaccaride,RSP)对猪卵母细胞体外成熟及发育潜力的影响。在猪卵母细胞成熟液中添加不同浓度的RSP,以排出第一极体作为核成熟的标志,观察卵母细胞核成熟情况,统计孤雌激活(PA)及体外受精(IVF)后胚胎的卵裂率和囊胚率,并检测卵母细胞内谷胱甘肽(GSH)的浓度及活性氧(ROS)水平。结果表明:与对照组(83.50%±1.00%)相比,添加0.6、6.0和60.0mg.L-1的RSP均能提高其核成熟率,但差异不显著(P>0.05);6.0和60.0mg.L-1的RSP组能显著提高卵母细胞内GSH含量(P<0.05),随着RSP浓度的增加可以降低ROS水平,但是各组之间差异不显著(P>0.05);孤雌激活中,60.0mg.L-1 RSP组的卵裂率(88.44%±5.06%)和6.0 mg.L-1 RSP的囊胚率(40.94%±8.23%)最高,且显著高于对照组(72.16%±7.38%和24.06%±5.16%,P<0.05);与对照组(33.52%±4.05%)相比,0.6、6.0和60.0mg.L-1RSP组体外受精卵裂率均显著提高(P<0.05),60.0mg.L-1 RSP组的囊胚率达(19.20%±3.13%),显著高于对照组的(9.60%±0.65%,P<0.05)。由此可见,在猪卵母细胞成熟过程中添加适当浓度的RSP,能提高其核成熟率,促进胞质成熟,从而提高其孤雌激活和体外受精后的胚胎发育能力。The study was designed to investigate the effect of Rhodiola sachalinensis polysaccaride （RSP） on developmental competence of porcine oocytes. Immature oocytes were matured in vitro and supplemented with different concentrations of RSP, the extrusion of first polar body was treated as a symbol of nuclear maturation, then the nuclear maturation rates, oocyte intracellular glutathione （GSH） content and reactive oxygen species （ROS） level, embryonic development after parthenogenetic activation （PA） and in vitro fertilization （IVF） were conducted. The result showed that the nuclear maturation rate in each RSP treatment were higher than that of control group （83.50±1.00%, P〉0.05）; The GV stage oocytes treated with 6.0 and 60.0 mg·L-1 RSP in vitro maturation medium could significantly increase the GSH content （P〈0.05）, and decrease the ROS level （P〉0.05）; After PA, the cleavage rate of 60.0 mg·L-1 RSP （88.44%! 5.06%） and the blastocyst rate of 6.0 mg·L-1 RSP （40. 94%±8. 23%） were the highest,and were significantly higher than those of control group （72.16±7.38 %, 24.06 ±5.16% ,P〈0.05） IVF cleavage rate in each RSP treatment was significantly increased （P;0.05）, IVF blastocyst rate （19.20±h3.13%） derived from oocytes treated with 60.0 mg·L-1 RSP was significantly higher than that of control group （9.60%±0.65%, P〈0.05）. The results showed that adding RSP in vitro maturation medium could increase nuclear maturation rate, promote cytoplasm maturation, and then increase the developmental competence of porcine oocytes after PA and IVF.

关 键 词：红景天多糖 卵母细胞 核成熟 谷胱甘肽 活性氧 孤雌激活 体外受精 猪 

分 类 号：S828.3[农业科学—畜牧学]