妊娠期关中奶山羊子宫中TLR4的定位和表达  

The Distribution and Expression of Toll-Like Receptor 4 in Pregnancy Uterus of Guanzhong Dairy Goat

作　　者：代盈盈[1] 王文丽[1] 赵慧英[1] 刘红改[1] 王薇[1] 

机构地区：[1]西北农林科技大学动物医学院,杨凌712100

出　　处：《畜牧兽医学报》2013年第4期629-634,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：抗病转基因牛新品种培育重点专项(2009ZX08007)

摘　　要：为了探讨Toll样受体4(Toll-like receptor 4,TLR4)在妊娠期山羊子宫中的分布和表达变化规律,通过免疫组织化学SP法及荧光定量RT-PCR技术,分别对妊娠早期(1～30d)、妊娠中期(31～120d)和妊娠晚期(121～150d)关中奶山羊子宫中TLR4的分布及TLR4mRNA的表达量的变化进行研究。结果表明,TLR4免疫反应阳性产物在关中奶山羊子宫的子宫内膜、肌层和浆膜均有不同程度分布,其中在子宫内膜上皮和腺上皮中阳性细胞最多、着色最深,肌层和浆膜层中免疫细胞着色较浅;随妊娠时期推移子宫内膜上皮细胞着色逐渐变浅,固有层中子宫腺上皮细胞着色先显著增强,到妊娠晚期又有所减弱;妊娠期子宫中TLR4相对表达量在妊娠期子宫内膜呈低-高-低的规律性变化,妊娠中期表达量极显著高于妊娠早期和晚期(P<0.01),妊娠早期表达量最低且极显著低于妊娠中期和晚期(P<0.01);在妊娠各期山羊子宫中的TLR4mRNA的表达量变化规律与TLR4相对表达量一致。表明TLR4在妊娠期关中奶山羊子宫的分布和表达具有一定的规律,并可能参与子宫的免疫和分泌调节功能。The aim of this study was to investigate the role of Toll-like receptor 4 （TLR4） in pregnancy uterus of goat. Immunochemistry SP method and fluorescent real-time quantitative PCR technology were used to study the distribution of TLR4 and the expression of TLR4 mRNA in Guanzhong dairy goat of early pregnancy （1-30 d）, midtrimester of pregnancy （31-120 d） and late pregnancy （121-150 d）. The results showed that TLR4 has different distribution in mucous layer, uterine muscle and uterine serosa of guanzhong goat uterus, TLR4 was mostly localized to the luminal and granular epithelium cells, there were less TLR4 positive staining cells in uterine muscle and uterine serosa; With the time of pregnancy increased endometrium epithelium cells staining gradually became lighter, the granular epithelium cells stained strongest in midtrimester of pregnancy, however, in late pregnancy the staining cells decreased; The relative expression of TLR4 exhibited low-high-low regularly change in endometrium, the level of TLR4 expression in midtrimester of pregnancy was significantly higher than that of TLR4 during other periods （P~ 0.01）, the level of TLR4 expression in early pregnancy was lowest, which was significantly low- er than that of TLR~ during other periods （P＇~ 0. 01）. TLR4 mRNA expressed in uterus throughout the whole pregnancy cycle, the expression level of TLR4 mRNA was consistent with the relative expression of TLR4. These results indicated that the distribution and expression in pregnancy uterus of Guanzhong dairy goat have certain rules, and that TLR4 may plays a role in regulating the immune and secretion of uterus.

关 键 词：妊娠子宫 TOLL样受体4 关中奶山羊 免疫组织化学SP法 荧光定量RT-PCR 

分 类 号：S852.1[农业科学—基础兽医学]