人牙周膜干细胞的成脂诱导实验研究  

Experiment on inducing human periodontal ligament stem cells into adipocytes

作　　者：钟志华[1] 鄢少君[2] 周先略[1] 贺国权[1] 何爱萍[1] 

机构地区：[1]荆州市中心医院口腔科,荆州434000 [2]长江大学医学院口腔科,荆州434000

出　　处：《口腔医学》2013年第4期254-257,共4页Stomatology

基　　金：湖北省卫生厅资助项目(2009-JX4B62)

摘　　要：目的体外分离培养成人的牙周膜干细胞(human periodontal ligament stem cells,hPDLSC),并进行成脂诱导实验。方法取新鲜拔除正畸牙的牙周膜为材料制成组织块,加入培养液分离培养,免疫磁珠法筛选人牙周膜干细胞,加入成脂诱导液诱导牙周膜干细胞向脂肪细胞转化。结果成功筛选人牙周膜干细胞,成脂诱导成功。结论免疫磁珠法是筛选人牙周膜干细胞的有效方法,人牙周膜干细胞可以向脂肪细胞分化。Objective To induce the periodontal ligament stem cells in vitro into adipocytes. Methods Periodontal ligament tissue was obtained from freshly extracted orthodontal teeth, then cultivated in nutrient solution, hPDLSC was selected with immunomagnetic beads method, hPDLSC was induced into adipocytes in the adipogenic nutrient solution. Results hPDLSC was selected successfully with immunomagnefie beads method, hPDLSC was induced into adipocytes successfully. Conclusions Immunomagnetic beads method is an effective way to isolate and purify the hPDLSC and hPDLSC was able to transfer into adipocyte.

关 键 词：人牙周膜干细胞 免疫磁珠 成脂诱导 

分 类 号：R349.5[医药卫生—基础医学]