东方田鼠非酒精性脂肪肝模型的建立  

Establishment of a Microtus fortis model of non-alcoholic fatty liver

作　　者：杨玉琴[1] 冯洁 柏熊 沈志敏 刘雄伟 高捷 谢建云 胡建华 高诚 

机构地区：[1]上海市公共卫生临床中心,上海201508 [2]上海市实验动物质量监督检验站 [3]上海实验动物研究中心,上海201203 [4]上海西普尔-必凯实验动物有限公司

出　　处：《中国实验动物学报》2013年第2期34-38,F0002,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：上海市科学基因资助项目(071409001;11140902801)

摘　　要：目的建立东方田鼠非酒精性脂肪肝模型,并观察测定其肝指数、病理、血清生化指标的动态变化。方法选取6周龄湖南洞庭湖种群雄性东方田鼠70只,随机分为2组,模型组饲喂高脂肪料,对照组饲喂高纤维料,分别于第2、4、6、8、12周处死,称量体重及肝重,计算肝指数,采血检测东方田鼠血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)、r-谷氨酰转移酶(r-GT)、胆碱酯酶(CHE)、甘油三酯(TG)、总胆固醇(TC)、游离脂肪酸(FFA)、葡萄糖(GLU)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL),并取肝脏HE染色后做病理学观察。结果与对照组相比,模型组6周时肝脏出现了典型的脂肪肝特征,肝重和肝指数都明显升高(P<0.05),血清ALT、AST、r-GT、CHE、TC、FFA、GLU和LDL都明显升高(P<0.05),HDL和TG均明显降低(P<0.05)。镜检观察到模型组田鼠肝细胞逐渐呈现弥漫性脂肪变性,6周时大范围出现脂滴,8周时肝内出现弥漫性大脂肪滴,12周后出现炎细胞的浸润。结论采用高脂饲料成功诱发东方田鼠非酒精性脂肪肝,可为研究非酒精性脂肪肝的发病机制和药物干预提供新的模型。Objective To establish a Microtusfortis model of non-alcoholic fatty liver and to observe its dynamic variation of hepatic, pathological and serum indexes. Methods Seventy 6-week-old male Microtus fortis were randomly divided into two groups. The voles of model group were fed high-fat ingredients and the voles of control group were fed high-fiber diet. The voles of each group were sacrificed at weeks 2, 4, 6, 8 and 12,respectively. The body weight and liver weight were measured, values of serum ALT, AST, ALP, r-GT, CHE, TG, TC, FFA, GLU, HDL and LDL were determined by a biochemical analyzer. The histological changes of livers were observed by light microscopy. Results The voles fed high-fat diet developed panlobular macrovesicular steatosis, whereas those fed high-fiber diet showed normal liv- er structure. Compared with the control group, liver weight and liver index of the model group were significantly increased, serum ALT, AST, r-GT, CHE, TC, FFA, GLU and LDL were significantly higher, and HDL and TG were significantly low- er （P 〈 0. 05）. Conclusions A Microtus fortls model of non-alcoholic fatty liver is successfully established by high-fat diet feeding for 6 weeks. It may provide a useful tool to elucidate the pathogenesis and treatment of nonalcoholic fatty liver disease.

关 键 词：东方田鼠 非酒精性脂肪肝 模型 

分 类 号：Q95-33[生物学—动物学]