山东地区汉族人群类风湿关节炎与CD14基因多态性的关联性  

Association between CD14 gene polymorphisms and rheumatoid arthritis in Han population of Shandong province

作　　者：李凤[1] 许菁[2] 郑加田[1] 张源潮[1] 孙红胜[1] 潘正论[1] 

机构地区：[1]山东大学附属省立医院风湿免疫科,济南250021 [2]莱芜市人民医院风湿免疫科,山东莱芜271100

出　　处：《山东大学学报（医学版）》2013年第4期87-91,95,共6页Journal of Shandong University:Health Sciences

基　　金：山东省中青年科学家科研奖励基金(2006BS03018);国家自然科学基金(30801025)

摘　　要：目的探讨山东地区汉族人群CD14基因rs2569190和rs2563298多态性与类风湿关节炎(RA)易感性的关联性。方法采用高分辨率熔解曲线方法对256例RA患者及331例健康对照者进行基因分型,计算各位点基因型及等位基因频率,检测血沉(ESR)、C反应蛋白(CRP)、抗环瓜氨酸多肽抗体(CCP)、葡萄糖6磷酸异构酶(GPI)等指标,比较两组各位点之间基因型及等位基因频率分布差异。结果 CD14基因rs2569190等位基因频率在RA组和对照组间差异有统计学意义(P=0.017),基因型分布在两组间差异无统计学意义(P=0.058);rs2563298基因型及等位基因频率在两组间差异无统计学意义(P=0.444,0.599)。经Bonferroni多重检验校正后,rs2569190等位基因频率在RA组与对照组间差异有统计学意义(校正后P=0.034)。CD14基因rs2569190和rs2563298在年龄、性别、病程、DAS28及各项指标上不同基因型间差异无统计学意义(P>0.05)。结论在山东地区汉族人群中CD14基因rs2569190多态性与类风湿关节炎易感性可能有关联。Objective To investigate the association between the polymorphisms of CD14 gene （rs2569190 and rs2563298） and rheumatoid arthritis （RA） susceptibility in Shandong Han population. Methods 256 cases with RA and 331 healthy controls were genotyped using high resolution melting （HRM） method. The genotype and allele fre- quencies were calculated, and the association between genotype and phenotype of RA were calculated and analyzed. ESR, CRP, CCP and GPI were detected. Results Significant difference was found in rs2569190 allele frequencies be- tween RA and controls （ P = 0. 017 ）, and there was no difference in genotype distribution between two groups （ P = 0. 058 ）. There was no difference in rs2563298 genotype distribution and allele frequencies between two groups （P = 0.444, 0. 599）. After Bonferroni multiple test correction, significant difference remained in rs2569190 allele frequen- cies between RA and controls （ adjusted P = 0. 034 ）. There were no differences of age, gender, disease duration, DAS28 level and other laboratory parameters among different genotypes of two SNPs. Conclusion The study showed that rs2569190 of CD14 gene polymorphism may play an important role in the susceptibility to RA in Shandong Han population.

关 键 词：CD14 基因多态性 类风湿关节炎 山东地区 汉族人群 

分 类 号：R593.2[医药卫生—内科学]