米诺环素对大鼠肢体缺血-再灌注继发肺损伤的影响  

The effect of minocycline on the lung injury after limbs ischemia-reperfusion in rats

作　　者：李郑琛[1] 贾英萍[1] 梁郑[1] 姜丽华[2] 

机构地区：[1]郑州市儿童医院麻醉科, 450000 [2]郑州大学第三附属医院麻醉科

出　　处：《临床麻醉学杂志》2013年第4期405-406,共2页Journal of Clinical Anesthesiology

摘　　要：目的探讨米诺环素对大鼠肢体缺血-再灌注继发肺损伤的影响。方法 SD成年雄性大鼠30只,随机分为三组(n=10),采用乙醚麻醉后,N组和M组大鼠经尾静脉注入生理盐水1.0ml,C组注入米诺环素10mg/kg。M组和C组分别结扎大鼠双下肢3h,再灌注4h制作缺血-再灌注模型。切除右肺,上叶测量湿干比,中叶测定IL-6含量;左肺下叶免疫组化S-P法观察基质金属蛋白酶(MMP)-2和MMP-9在肺组织的着色及灰度对比。结果右肺上叶湿干比、左肺下叶MMP-2和MMP-9表达以及匀浆IL-6含量均为M组>C组>N组(P<0.01)。结论米诺环素可通过抑制炎性反应减轻大鼠肢体缺血-再灌注所造成的肺损伤。Objective To explore the effect of minocyeline in lung tissue after limbs ischemia- reperfusion in rats. Methods Thirty healthy SD rats were randomly divided into 3 groups （n= 10） ： group M,group N and group C. Group N and group M received iv. normal sodium 1.0 ml. Group C received iv. minocycline 10 rng/kg （about 1.0 ml）. Group M and group C received lower limb ligation for 3 h and reperfusion for 4 h. Right lung wet-dry ratio, levels of MMP-2, MMP-9 and IL-6 were investigated in the left lung. Results The w/d ratio, the expressions of MMP-2, MMP-9, and the level of tissue IL-6 was increased by limb ischemia-repeffusion with the sequence group M ~ C ~ N （P ~ 0.01 ）. Conclusion Minocycline can protect the lung against ischemia-repeffusion injury by inhibiting inflammatory response.

关 键 词：米诺环素 肺损伤 基质金属蛋白酶-2 基质金属蛋白酶-9 白细胞介素-6 

分 类 号：R965[医药卫生—药理学]