液体培养法联合菌种鉴定技术诊断HIV与分枝杆菌双重感染  

The diagnosis of Mycobacterial coinfection among HIV/AIDS patients by combining liquid culture with strain identification technique

作　　者：曾昭瑛[1] 赵秀英[2] 

机构地区：[1]首都医科大学附属北京友谊医院临床检验中心,北京100050 [2]首都医科大学附属佑安医院临床检验中心

出　　处：《中国防痨杂志》2013年第4期260-263,共4页Chinese Journal of Antituberculosis

摘　　要：目的利用分枝杆菌液体培养法联合菌种鉴定技术诊断HIV与分枝杆菌双重感染,以了解目前华北地区HIV与分枝杆菌双重感染的情况。方法对2009—2011年收治的53例疑似HIV与分枝杆菌双重感染患者的可疑感染部位标本进行分枝杆菌液体培养及菌种鉴定。收集所有经确诊为HIV与分枝杆菌双重感染患者的临床资料、影像学及相关实验室检查结果进行分析与总结。结果53例疑似HIV与分枝杆菌双重感染的患者中,病原学证实存在分枝杆菌感染的患者为19例,11例(57.9%)为单纯肺脏累及,2例(10.5%)为肺外累及,4例(21.1%)为分枝杆菌血症,2例(10.5%)为肺脏累及和分枝杆菌血症并存。16例患者为结核分枝杆菌感染,3例患者为鸟胞内分枝杆菌复合体感染。15例(78.9%,15/19)患者CD4+T淋巴细胞计数<100个/μl。结论联合应用液体培养法与菌种鉴定技术对于诊断HIV与分枝杆菌双重感染是有效的,值得在临床推广应用。Objective To evaluate the prevalence of HIV/Mycobacteria coinfection with liquid Mycobacteria culture and molecular technique in North China. Methods Fifty-three suspected HIV/AIDS patients were per- formed with liquid culture and strain identification of Mycobacteffa from 2009 to 2011. The clinical manifestation, ra- diological imaging and relevant laboratory data from all patients with definite diagnosis of HIV/Mycobacteria coinfec- tion were analyzed. Results Of 19 patients confirmed with HIV/Mycobacteria coinfeetion, Mycobacteriosis involved in pulmonary only in 11（57.9%）, extra-pulmonary （brain and liver） in 2（10.5%）, Mycobacteremia in 4（21.1%）, both pulmonary and mycobacteremia in 2 （10.5%）. Mycobacterium lubeculosis （ 16,84.2%） and Mycobacterium avi- um-intracellulare complex （3,15.7%）were isolated from the 19 patients. 15 （78.9 % ） patients had a quite low CD4 ＋ T lymphocytes count less than 100 cells per microlitre in peripheral blood. Conclusions Combining liquid culture and molecular techniques is effective method to identify HIV/Mycobacteria coinfection and to be applied in the clinical practice.

关 键 词：重叠感染 HIV感染 分枝杆菌感染 分枝杆菌属 细菌学技术 培养基 

分 类 号：R446.6[医药卫生—诊断学]