毛冬青注射液细胞毒性及类过敏反应的评价  

Study of Cytotoxicity and Anaphylactoid Reaction of Ilex Pubescens Hook Injections

作　　者：范能全 杨柳 詹宇杰 张璞 

机构地区：[1]重庆市食品药品检验所,重庆401121

出　　处：《中国药师》2013年第4期524-525,共2页China Pharmacist

摘　　要：目的:研究毛冬青注射液的安全性,为临床合理用药提供参考。方法:通过L-929细胞毒性实验和RBL-2H3细胞脱颗粒实验,考察毛冬青注射液的临床毒性大小以及是否存在类过敏的不良反应。结果:毛冬青注射液在高浓度时(333μl.ml-1)抑制细胞增殖,低浓度时(111μl.ml-1)促进细胞增殖,并有浓度依赖性,同时会引起部分RBL-2H3细胞脱颗粒。结论:细胞毒性实验可用于评价药物的毒性大小;细胞脱颗粒实验可用于评价药物的类过敏反应。Objective： To evaluate the safety of llex Pubescens Hook injections in order to provide the references for the rational clinical use. Method： The clinical toxicity and negative anaphylactoid reactions of the injections were studied using L-929 cytotoxicity experiment and RBL-2H3 cell degranulation experiment. Result： The results showed that the cell proliferation was dependent on the concentration of the injections, namely reduced by the higher concentration （333 μl.ml^-1）while induced by the lower concentration （111 μl.ml^-1）. Some RBL-2H3 cells were degranulated by the injections. Conclusion： The cytotoxicity experiment can be used to judge the toxicity of medicines, and the cell degranulation experiment can evaluate the anaphylactoid reactions.

关 键 词：毛冬青注射液 细胞毒性 类过敏反应 细胞脱颗粒 

分 类 号：R965.3[医药卫生—药理学]