过敏性哮喘患者IL-4和IL-13以及EBV抗体的检测及临床意义  

DETECTION AND SIGNIFICANCE OF IL-4 AND IL-13 AND EBV ANTIBODY IN PATIENT WITH ALLERGIC ASTHMA

作　　者：颜廷斐[1] 阎丽萍[2] 曲松本[2] 

机构地区：[1]青岛大学医学院附属医院检验科,山东青岛266003 [2]青岛大学医学院第二附属医院检验科,山东青岛266042

出　　处：《医学检验与临床》2013年第1期16-18,23,共4页Medical Laboratory Science and Clinics

摘　　要：目的：探讨人血清IL-4和IL-13水平以及EBV感染在过敏性哮喘发生中的作用，分析过敏性哮喘患者EBV感染与患者血清IL-4和IL-13水平的相互关系。方法：采用酶联免疫吸附试验（ELISA）检测30例过敏性哮喘患者和4f）例健康对照组血清IL-4和IL-13含量以及血清EB病毒早期抗原抗体EA—IgM和EA—IgAvT,及衣壳抗原抗体VCA—IgM，VCA-IgA和VCA—IgG水平，并进行相关性研究。结果：过敏性哮喘患者血清IL-4（11．00±5．72pg／1n1），IL-13（4．53±2．22pg／m1），EA—IgM（3．86±1．25U／m1），EA—IgA（1．71±0．64U／m1）和VCA—IgM（8．93±3．03U／m1）明显高于正常对照组（3．88±1．73pg／ml，2．87±1．07pg／ml，2．68±1．12U／ml，1．29±0．29U／ml和561±1．95U／ml；P〈0．001，P〈0．01，P〈0．01，P〈0．05和P〈0．001）。过敏性哮喘患者血清VCA—IgA和VCA—IgG水平分别为13．29±23．45u／m1和8．01±19．30U／ml，对照组血清VCA—IgA和VCA—IgG水平分别为135．43±102．79U／ml和121．48±122．11U／ml），统计学分析表明两组间无显著性差异（P均〉0．05）。患者血清IL-4和IL-13水平与EBV抗体EA—IgM，EA—IgA，VCA—IgM，VCA-IgA，以及VCA-IgG水平无明显相关关系（P均〉0．05）。结论：过敏性哮喘的发生发展可能与血清IL-4和I-一13水平升高有关；过敏性哮喘患者存在EBV的活动性感染；EBV感染可能不是导致过敏性哮喘患者血清IL-4和IL-13水平升高的影响因素。Objective ： To investigate the association of IL-4 and IL-13 level in serum and EBV infection in the development of Allergic Asthma. To analysis the correlations between EBV infection and IL-4 and IL-13 level in serum in patients with Allergic Asthma.Methods ： The serum contents of IL-4 and IL-13 and the serum levels of antibodies against EBV early antigen EA-IgM and EA-IgA and viral capsid antigen VCA-lgM,VCA-IgA and VCA-IgG of 30 allergic asthma patients and 40 healthy controls were detected using ELISA. Results ： The serum levels of 1L-4, IL-13, EA-IgM, EA-IgA and VCA-IgM in the Allergic Asthma group were significantly higher than that in the control group（P〈0.001,P〈0.01,P〈0.01,P〈0.05 and P〈0.001）. The EBV VCA- IgA and VCA-lgG levels of the allergic asthma group and control group in serum were 13.29±23.45U/ml and 8.01±19.30U/ml, 135.43±102.79U/ml and 121.48±122.11U/ml respectively, there was not significantly difference between two groups.There was no association between IL-4, IL-13 and EBV EA-IgM, EA-IgA,VCA-IgM, VCA-IgA and VCA-IgG in patients with Allergic Asthma （P〉0.05）. Conclusions ： The occurrence and development of Allergic Asthma was probably concerned with the increased expression oflL-4 and IL-13 in serum. The EBV active infection was exist in Allergic Asthma patients.The EBV infection may not be influencing factor what result in the increased expression oflL-4 and IL-13 in serum in Allergic Asthma patients.

关 键 词：过敏性哮喘 白细胞介素4 白细胞介素13 EB病毒抗体 

分 类 号：R562.25[医药卫生—呼吸系统]