加工方法对百合质量的影响研究  

Study on the Effect of Different Processing Methods in Lilium lancifolium Thunb. Bulbs

作　　者：聂慧[1,2] 严辉[2] 钱大玮[2] 段金廒[2] 欧阳臻[1] 钱叶飞[2] 管汉亮[2] 

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]南京中医药大学江苏省方剂高技术研究重点实验室,江苏南京210046

出　　处：《中国现代中药》2013年第4期308-313,共6页Modern Chinese Medicine

基　　金：国家中医药管理局行业科研专项(201107009)

摘　　要：目的:研究加工方法对百合质量的影响,为百合加工方法的规范化提供参考依据。方法:新鲜百合样品分别采用煮制和蒸制2种烫片方法、不同干燥方法(包括热风烘干、微波干燥、远红外干燥和冷冻干燥)进行加工,样品中酚性甘油酯类、核苷、氨基酸及多糖等成分分别采用超高效液相-二极管阵列检测器(UPLC-DAD)、超高效液相-三重四级杆质谱联用仪(UPLC-TQ/MS)及紫外-可见分光光度法(UV)测定。结果:烫片过程,煮5～8min和蒸8～10min样品不仅外观色泽较佳,而且各成分含量较高;干燥方法,60～80℃烘干和冷冻干燥法较好。结论:烫片是百合干加工过程中必不可少的环节;确定最佳工艺条件为煮5～8min,60～80℃烘干或冷冻干燥。The purpose of this paper is to give support to establishing a standard processing method for Lilium lancifolium Thunb. bulbs, by comparing the changes on chemical compositions via different processes. Steam and boiling water was used in processing fresh bulbs. And 4 kinds of drying methods were used to deal with the boiled samples, including heated air drying, microwave drying, far infrared drying and freeze drying. Ultra-high performance liquid chromatography along with a diode array detector （UPLC-DAD） was used for analyzing phenylpropenoid glycosides, ultra-high performance liquid chromatography combined with a triple quadrupole electrospray tandem mass spectrometry （UPLC-TQ/MS） was applied for measuring nucleosides and amino acids, UV spectrophotometry （UV） was used for determining polysaccharide, respectively. It was in good appearance and higher contents that the samples were steamed 8 - 10 min or boiled 5 - 8 min, heated air dried at 60 - 80 ℃ and freeze dried. It was concluded that the boiling process was absolutely necessary for the primary processing. With considering various factors, the optimal conditions were obtained as following： the boiling time was 5 - 8 minutes, and the drying method was heated air drying at 60 - 80℃ or freeze drying.

关 键 词：百合 烫片 干燥 工艺优化 

分 类 号：R282.71[医药卫生—中药学]