外周血肿瘤坏死因子-α信使核糖核酸表达及与核因子-κB活化与慢性心力衰竭严重程度的临床研究  

Clinical Investigation of Peripheral Blood TNF-α mRNA Expression and Nuclear Factor-κΒ Activation on Severity of Congestive Heart Failure

作　　者：杨丽霞[1] 吕晋琳[1] 齐峰[1] 叶金善[1] 郭瑞威[1] 石燕昆[1] 

机构地区：[1]云南省昆明市成都军区昆明总医院心血管内科,650032

出　　处：《中国循环杂志》2013年第2期122-124,共3页Chinese Circulation Journal

摘　　要：目的 :探讨肿瘤坏死因子-α信使核糖核酸(TNF-αmRNA)表达与核因子-κB(NF-κB)活化在慢性心力衰竭(CHF)心肌重塑中的意义。方法 :CHF患者76例为CHF组,健康体检者30例为对照组,提取外周血单个核细胞(PBMCs),采用酶联免疫吸附法(ELISA)检测其NF-κB的水平,用逆转录-聚合酶链反应(RT-PCR)方法检测TNF-αmRNA的表达,并与心功能分级、心脏超声相关参数比较。结果 :CHF组较对照组外周血单个核细胞NF-κB水平和TNF-αmRNA表达显著增高(P<0.05),且CHF组的两指标之间呈显著正相关(P<0.01),且两者分别与心脏收缩功能指标呈显著负相关(P<0.01),而与心肌重塑指标呈显著正相关(P<0.01)。结论 :CHF患者外周血单个核细胞NF-κB的活化促进TNF-αmRNA表达增强可能是CHF时心功能恶化和心肌重塑进展的原因之一。Objective： To investigate peripheral blood tumor necrosis factor- α（TNF-α） mRNA expression and nuclear factor- κB （NF- κB） activation on severity of congestive heart failure （CHF）. Methods： Our study included 2 groups, CHF group, n=76 patients and Control group, n=30 healthy subjects. The activity of NF- κB in peripheral blood mononuclear cells was examined by ELISA, and the expression of TNF α -mRNA was measured by RT-PCR. The echocardiography parameters and cardiac function indexes were compared between 2 groups. Results： Compared with Control group, CHF group presented increased NF-κB activity and TNF-α mRNA expression, P〈0.05. In CHF group, NF-κB activity and TNF-α mRNA expression showed positive correlation, P〈0.01, and both of them negatively related to cardiac functional parameters, P〈0.01, and positively related to cardiac remodeling indexes, P〈0.01. Conclusion： The activation of NF-κB of peripheral blood mononuclear ceils in CHF patients promoted TNF-α mRNA expression, which might be one of the reasons for reducing cardiac function and cardiac remodeling during CHF period.

关 键 词：慢性心力衰竭 核因子-ΚB 肿瘤坏死因子-Α 

分 类 号：R54[医药卫生—心血管疾病]