血液标本在全自动血培养系统的临床应用探讨  

Blood Specimen in Fully Automatic Blood Culture System Discussed the Clinical Application

作　　者：丁世才 

机构地区：[1]云南曲靖市妇幼医院检验科,曲靖655000

出　　处：《航空航天医学杂志》2013年第4期408-410,共3页Journal of Aerospace medicine

摘　　要：目的:通过对血液样本在全自动血培养系统的培养结果,探讨全自动血培养系统在血液感染的微生物种类以及构成的临床应用价值。方法:用BD9120全自动血培养系统检测7 289份血液标本,对检出阳性的时间、阳性率、假阳性、假阴性和细菌种类进行评估分析。结果:血液样本中最快阳性检出时间为2.13 h,在12 h、24 h、48 h、72 h的阳性检出率分别为37.7%、61.0%、82.1%、96.3%。假阳性率为1.0%,假阴性率为0.2%。分离出19属,19种,共1126株病原微生物,阳性率为15.4%,其中革兰氏阳性球菌632株(56.1%),革兰氏阴性杆菌326(29.0%)占总检出率的85.1%。结论:全自动血培养系统的临床应用提高了病原菌检出的阳性率,缩短了阳性检出时间,检出细菌种类多,为败血症及严重感染的诊断和治疗提供快速准确的诊断依据。Abstract Objective：Through the blood sample in fully automatic blood culture system training results, this paper dis- cusses the automatic blood culture system in blood infection of microbial species composition and the clinical application value. Methods： Using BD9120 automatic blood culture system testing of 7289 blood specimens, the detection positive time, positive rate and false positive and false negative and bacteria species evaluation analysis. Results： Blood sample fastest positive detection time is 2. 13 h, in 12 h, 24 h, 48 h, 72 h positive detection rate were 37.7%, 61.0%,82. 1%, 96. 3%. False positive rate was 1.0%, the false negative rate was 0. 2%. Separate the 19 genera and 19 species, a total of 1126 strains of pathogenic microorganism, positive rate was 15.4%, among which gram positive cocci 632 strains （56. 1%）, gram negative bacilli 326 （29.0%） accounted for 85.1% of the total prevalence rate. Conclusions：Fully automatic blood culture system to improve the clinical application of the pathogen detection of positive rate, shorten the positive detection time, detection more kinds of bacteria for sepsis and the diagnosis and treatment of severe infection provides a rapid and accurate diagnosis basis.

关 键 词：血液样本 全自动血培养系统 假阳性 革兰阴性 

分 类 号：R446.1[医药卫生—诊断学]