同步扫描荧光光谱对水体四种重金属离子毒性的研究  

Studies on Toxicity of Four Kinds of Heavy Metals in Water by Synchronous-Scan Fluorescence

作　　者：段静波[1] 刘文清[1] 张玉钧[1] 赵南京[1] 王志刚[1] 殷高方[1] 方丽[1] 刘晶[1] 

机构地区：[1]中国科学院环境光学与技术重点实验室,中国科学院安徽光学精密机械研究所,安徽合肥230031

出　　处：《光谱学与光谱分析》2013年第5期1262-1265,共4页Spectroscopy and Spectral Analysis

基　　金：国家高技术研究发展计划(863计划)项目(2009AA063005);安徽省杰出青年科学基金项目(1108085J19);安徽省自然科学基金项目(11040606M26);中国科学院安徽光学精密机械研究所所长基金项目(Y03AG31144)资助

摘　　要：采用三维荧光光谱和Δλ=20nm同步扫描荧光光谱技术研究Hg2+,Cd2+,Cu2+,Zn2+胁迫作用下蛋白核小球藻(Chlorella pyrenoidosa)荧光光谱的变化情况,两种光谱技术得到了较为一致的结论:即胁迫作用96h后,蛋白核小球藻荧光中心处的荧光值显著降低,四种重金属离子对藻细胞光合系统的叶绿素a和叶绿素b造成了严重的破坏。进一步分析四种重金属离子浓度与由同步扫描荧光光谱得到的藻荧光猝灭效率I0/I的相关性可知:重金属离子的毒性与荧光猝灭效率I0/I呈正相关关系,均随重金属离子浓度和胁迫时间的增加而增强。对某一种重金属而言,同步扫描荧光光谱可实现藻细胞对该重金属毒性的评价。与三维荧光光谱相比,同步扫描荧光光谱具有耗时少、对特定荧光中心选择性高等优点,是研究水体有毒污染物毒性的有利工具。Spectrofluorometry of chlorella pyrenoidosa was studied by three dimensional excitation-emission （3DEEM） fluores- cence spectroscopy and synchronous scan fluorescence spectroscopy with Δλ=20 nm in the stress of Hg2＋ , Cd2＋ , Cu2＋ and Zn2＋. The conclusion from two kinds of Spectrofluorometry was the same., after 96h stress by heavy metals, the maximum fluo- rescence values reduced obviously, chlorophyll-a and chlorophyll-b in the photosynthetic system were seriously damaged by heavy metal. Further analysis of the correlations between heavy metal concentration and fluorescence quenching efficiency I0/I can con- clude that the toxicity of heavy metal and the fluorescence quenching efficiency I0/I were positively correlated, and they all in- creased with the heavy metal concentration and stress time. For one kind of heavy metals, synchronous scan fluorometry is a sen- sitive method for its toxicity assessment. Compared to 3DEEM fluorescence spectroscopy, synchronous scan fluorescence spec- troscopy is less time consuming and of higher selectivity. It is suitable to assess the toxicity of pollutions in water.

关 键 词：蛋白核小球藻 同步扫描荧光光谱 重金属胁迫 

分 类 号：X524[环境科学与工程—环境工程]