连翘和美国金钟连翘的抗寒性分析  

Study on cold resistance for Forsythia suspensa and F. intermelia

作　　者：回彦哲[1] 袁小亚[1] 牛芳芳[1] 王阿丽[1] 闫华元 任士福[1] 

机构地区：[1]河北农业大学林学院,河北保定071000 [2]井陉县林业局,河北井陉050300

出　　处：《河北农业大学学报》2013年第2期36-39,共4页Journal of Hebei Agricultural University

基　　金：河北省教育厅项目(ZH2012076);河北省林业局项目(1116409)资助

摘　　要：对低温胁迫下连翘[Forsythia suspensa(Thunb.)Vahl]和美国金钟连翘(F.intermelia Zabel)枝条梢部、中部和基部的电导率、SOD酶活性和可溶性蛋白质含量进行了研究,结果表明:随着温度的降低,可溶性蛋白含量呈明显的先上升后下降的变化趋势,与抗寒性相关性较好;SOD酶活性在低温胁迫初期有上升趋势但不明显,基本呈逐渐降低的趋势,与抗寒性相关性较差,在连翘抗寒性鉴定中,不宜单独使用。2种连翘枝条各部分的半致死温度分别为:连翘枝条梢部-14.13℃,连翘枝条中部-16.51℃,连翘枝条基部-17.59℃;美国金钟连翘枝条梢部-14.26℃,枝条中部-17.12℃,枝条基部-18.78℃。2种连翘相比,无论梢部、中部、还是基部,美国金钟连翘抗寒能力均强于连翘。与中部、梢部相比,2种连翘基部抗寒性差异较大。Forsythia suspensa Vabl. and F. intermedia were used to investigate the effects of chilling stress on the conductivity, SOD activity, soluble protein content changes. The results showed that, as the temperature decreases, the soluble protein content increased and then de- creased and had a good correlation with the cold hardiness. The upward trend of SOD activity was not obvious, substantially gradually decreased, and had a poor correlation with the cold hardiness, indicating that SOD activity should not be used alone in the forsythia cold resist- ance identification. The semi-lethal temperature of the two forsythia＇s various parts： The tip of F. suspensa --14.13℃ ,The central of F. suspensa--16.51 ℃ ,the base of F. suspensa --17.59 ℃, The tip of F. intermedia --14.26 ℃,The central of F. intermedia --17.12℃ ,The base of F. inter- media --18.78℃ In contrast, regardless of the tip, the central and the base, F. interrnedia were more hardy than F. suspensa . Compared with the top and central tip, cold hardiness of the base was significant difference.

关 键 词：连翘 美国金钟连翘 可溶性蛋白 SOD 电导率 抗寒性 

分 类 号：S685.24[农业科学—观赏园艺]