甘草无菌苗茎尖快繁技术研究  

Study of rapid reproduction technique on germfree plants shoot-tip of Glycyrrhiza

作　　者：汪连海[1] 蔺海明[1,2] 张汉平 王升元 邱黛玉[1,2] 柳福智[1,2] 闫立本 赵贵亮 

机构地区：[1]甘肃农业大学农学院,甘肃兰州730070 [2]酒泉甘草生物育种研究所,甘肃酒泉733101 [3]甘肃省酒泉航天育种示范基地,甘肃酒泉733101

出　　处：《河北农业大学学报》2013年第2期40-45,共6页Journal of Hebei Agricultural University

基　　金："十二五"国家科技支撑计划课题(2011BAI05B01);甘肃省农牧厅科技创新项目(GNCX-2011-37);甘肃省教育厅基金项目(0902-03)资助

摘　　要：以甘草无菌苗茎尖为材料,采用5因素4水平正交试验,以诱导率、不定芽数、叶片数、株高和鲜重为指标,研究6-BA、KT、NAA、蔗糖和不同培养基对甘草无菌苗茎尖诱导的影响,并采取排队评分与公式评分法对几个指标打分后进行分析,优化出最佳的激素配比,建立甘草茎尖快繁体系。结果表明:不同培养基对甘草茎尖诱导苗的影响最大,6-BA对甘草茎尖诱导苗的生长发育影响次之,蔗糖对甘草茎尖诱导苗生长发育影响最小,甘草茎尖诱导苗最佳激素配比为:MS+6-BA 0.3mg/L+NAA 1.0mg/L+KT 0.3mg/L+蔗糖30g/L;增殖培养基MS+6-BA1.0mg/L+NAA0.1mg/L+蔗糖30g/L,增殖倍数为7倍,平均株高为5.4cm;生根培养基MS+KH2PO413.5mg/L+蔗糖30g/L,生根率达到100%。This experiment used the material of GZycyrrhiza uralensis Fisch. shoot-tips and took the method of orthogonal experiment of 5 factors and 4 levels to research the 6-BA.KT. NAA, sucrose and different medium＇s induced influence on germfree plants shoot-tip of Glycyr- rhiza. Used line score and formula scoring methods to analyse several index after grading, opti- mized the best ratio of hormone to establish the rapid propagation system of shoot-tips. Results showed that culture mediums had the greatest impact, 6-BA was the minor, Sucrose had the least impact. The suitable hormone proportion of culture medium for Glycyrrhiza shoot-tip was MS＋6-BA0.3 mg/L＋NAA1.0mg/L＋KT0.3 mg/L＋sucrose 30 g/L. The suitable consists of proliferation medium was MS＋6--BA1.0 mg/L＋NAA0.1 mg/L＋sucrose30 g/L,prolifer- ation ratio reached 7times, average plant height was 5.4 cm;The suitable consists of rooting medium was MS＋KH2PO413.5 mg/L＋sucrose 30 g/L and rooting rate reached 100%.

关 键 词：甘草 无菌苗 快繁 茎尖 

分 类 号：S567.71[农业科学—中草药栽培]