白细胞介素-8在子宫内膜异位症中的作用  

Effect of IL-8 on pathogenesis of endometriosis

作　　者：孙琳[1] 王健[2] 

机构地区：[1]安徽理工大学医学院妇产科护理学教研室,淮南232001 [2]安徽理工大学医学院病原学与免疫学教研室

出　　处：《现代预防医学》2013年第9期1634-1637,共4页Modern Preventive Medicine

基　　金：安徽省自然科学基金项目(090413138);安徽省教育厅自然科学基金项目(KJ2008A153)

摘　　要：目的探讨白细胞介素-8(Interleukin-8,IL-8)在子宫内膜异位症中的表达及其发病机制中的作用。方法将内异症患者按R-AFS进行分期,分别用ELISA检测腹腔中IL-8表达水平,Real-time-PCR检测内膜组织IL-8mRNA水平,MTT法测定经IL-8、anti-IL-8刺激后子宫内膜基质细胞的增殖和抑制情况。结果内异症患者与对照组腹腔液中IL-8的表达水平分别为(23.37±8.23)pg/ml、(12.32±4.27)pg/ml,差异有统计学意义(P﹤0.05),内异症增生期和分泌期内膜组织的IL-8mRNA表达水平(log cDNA/logGAPDH)分别为1.3011±0.2689、1.1239±0.2738,两者差异无统计学意义(P﹥0.05)。体外培养中,IL-8对内膜基质细胞增殖有明显的剂量和时间依赖性,且在浓度为10ng/ml时最显著,IL-8对基质细胞的增殖作用可被anti-IL-8抑制。结论 IL-8作为自分泌生长因子和正反馈因子,直接促进内膜基质细胞生长,在内异症的发生和发展中起重要作用。OBJECTIVE To explore the level and effect of IL-8 on pathogenesis of endometriosis.METHODS The patients were divided in different group on base of R-AFS standard,and the levels of IL-8 in peritoneal fluid and IL-8 mRNA in endomembrane tissue were detected by ELISA,real-time-PCR,respectively.The levels of and the effect of IL-8,anti-IL-8 for hyperplasy or restrain of stroma cells were detected by MTT.RESULTS The levels of IL-8 in peritoneal fluid and endomembrane tissue were（23.37 ± 8.23）pg/ml,（12.32 ± 4.27）pg/ml,respectively（P﹤0.05）.However,the expression of IL-8 mRNA in estrin phase of endomembrane was 1.301 1 ± 0.268 9（log cDNA/log GAPDH）,1.123 9 ± 0.273 8（log cDNA/log GAPDH）.It was similar in two groups（P﹥0.05）.The dose-time dependence was expressed between the level of IL-8 and hyperplasy of stroma cells,and the best concentration was at site of 10ng/ml.The proliferative effect of IL-8 on stroma cell was inhibited by anti-IL-8.CONCLUSION IL-8,a kind of autocrine growth factor and positive feedback factor,can promote the growth of stroma cells and play a key role on pathogenesis of endometriosis.

关 键 词：子宫内膜异位症 腹腔液 IL-8 IL-8MRNA 内膜组织 

分 类 号：R711.71[医药卫生—妇产科学]