Metabolomic Profiling in Selaginella lepidophylla at Various Hydration States Provides New Insights into the Mechanistic Basis of Desiccation Tolerance  

作　　者：Abou Yobi Bernard W.M. Wone Wenxin Xu Danny C. Alexander Lining Guo John A. Ryals Melvin J. Oliver John C. Cushman 

机构地区：[1]Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557-0330, USA [2]Department of Biological Sciences, University of Nevada, Reno, NV 89557-0314, USA [3]Metabolon Inc., 800 Capitola Drive, Suite 1, Durham, NC 27713, USA [4]US Department of Agriculture-Agricultural Research Service, Plant Genetic Research Unit, University of Missouri, Columbia, MI 65211, USA

出　　处：《Molecular Plant》2013年第2期369-385,共17页分子植物（英文版）

摘　　要：Selaginella lepidophylla is one of only a few species of spike mosses （Selaginellaceae） that have evolved des-iccation tolerance （DT） or the ability to ＇resurrect＇ from an air-dried state. In order to understand the metabolic basis of DT, S, lepidophylla was subjected to a five-stage, rehydration/dehydration cycle, then analyzed using non-biased, global metabolomics profiling technology based on GC/MS and UHLC/MS/MS2 platforms. A total of 251 metabolites including 167 named （66.5%） and 84 （33.4%） unnamed compounds were characterized. Only 42 （16.7%） and 74 （29.5%） of compounds showed significantly increased or decreased abundance, respectively, indicating that most compounds were produced con-stitutively, including highly abundant trehalose, sucrose, and glucose. Several glycolysis/gluconeogenesis and tricarboxylic acid （TCA） cycle intermediates showed increased abundance at 100% relative water content （RWC） and 50% RWC. Vanillate, a potent antioxidant, was also more abundant in the hydrated state. Many different sugar alcohols and sugar acids were more abundant in the hydrated state. These polyols likely decelerate the rate of water loss during the drying process as well as slow water absorption during rehydration, stabilize proteins, and scavenge reactive oxygen species （ROS）. In contrast, nitrogen-rich and y-glutamyl amino acids, citrulline, and nucleotide catabolism products （e.g. allantoin） were more abundant in the dry states, suggesting that these compounds might play important roles in nitrogen remobilization during rehydration or in ROS scavenging. UV-protective compounds such as 3-（3-hydroxyphenyl）propionate, apigenin, and naringenin, were more abundant in the dry states. Most lipids were produced constitutively, with the exception of choline phosphate, which was more abundant in dry states and likely plays a role in membrane hydration and stabilization. In contrast, several poly- unsaturated fatty acids were more abundant in the hydrated states, suggestinSelaginella lepidophylla is one of only a few species of spike mosses （Selaginellaceae） that have evolved des-iccation tolerance （DT） or the ability to ＇resurrect＇ from an air-dried state. In order to understand the metabolic basis of DT, S, lepidophylla was subjected to a five-stage, rehydration/dehydration cycle, then analyzed using non-biased, global metabolomics profiling technology based on GC/MS and UHLC/MS/MS2 platforms. A total of 251 metabolites including 167 named （66.5%） and 84 （33.4%） unnamed compounds were characterized. Only 42 （16.7%） and 74 （29.5%） of compounds showed significantly increased or decreased abundance, respectively, indicating that most compounds were produced con-stitutively, including highly abundant trehalose, sucrose, and glucose. Several glycolysis/gluconeogenesis and tricarboxylic acid （TCA） cycle intermediates showed increased abundance at 100% relative water content （RWC） and 50% RWC. Vanillate, a potent antioxidant, was also more abundant in the hydrated state. Many different sugar alcohols and sugar acids were more abundant in the hydrated state. These polyols likely decelerate the rate of water loss during the drying process as well as slow water absorption during rehydration, stabilize proteins, and scavenge reactive oxygen species （ROS）. In contrast, nitrogen-rich and y-glutamyl amino acids, citrulline, and nucleotide catabolism products （e.g. allantoin） were more abundant in the dry states, suggesting that these compounds might play important roles in nitrogen remobilization during rehydration or in ROS scavenging. UV-protective compounds such as 3-（3-hydroxyphenyl）propionate, apigenin, and naringenin, were more abundant in the dry states. Most lipids were produced constitutively, with the exception of choline phosphate, which was more abundant in dry states and likely plays a role in membrane hydration and stabilization. In contrast, several poly- unsaturated fatty acids were more abundant in the hydrated states, suggestin

关 键 词：abiotic/environmental stress mass spectrometry metabolomics oxidative/photooxidative stress desiccationtolerance Selaginella. 

分 类 号：TQ533.2[化学工程—煤化学工程] P588.22[天文地球—岩石学]