Expression of a microRNA-Resistant Target Transgene Misrepresents the Functional Significance of the Endogenous microRNA： Target Gene Relationship  

作　　者：Junyan Li Anthony A. Millar 

机构地区：[1]Research School of Biology, Australian National University, 0200 ACT, Australia

出　　处：《Molecular Plant》2013年第2期577-580,共4页分子植物（英文版）

摘　　要：Dear Editor, A current major challenge in microRNA （miRNA） biology is the elucidation of their functions. A key first step in this pur-suit is the identification of functionally relevant miRNA：target gene relationships. Although the molecular verification of miRNA targets with degradome or 5＇-end RACE approaches has been very successful in plants, the detection of miRNA-guided cleavage products may not be reflective of functional importance （Allen et al., 2010）, which can only be definitively defined by genetic approaches.Dear Editor, A current major challenge in microRNA （miRNA） biology is the elucidation of their functions. A key first step in this pur-suit is the identification of functionally relevant miRNA：target gene relationships. Although the molecular verification of miRNA targets with degradome or 5＇-end RACE approaches has been very successful in plants, the detection of miRNA-guided cleavage products may not be reflective of functional importance （Allen et al., 2010）, which can only be definitively defined by genetic approaches.

关 键 词：MICRORNA 靶基因 内源性 转基因 miRNA 小分子RNA 识别功能 分子验证 

分 类 号：Q522[生物学—生物化学] Q78