脂联素通过增强线粒体生物合成和功能减轻高糖/高脂诱导的心肌细胞损伤  

Adiponectin exerts cardioprotective effects against high-glucose/high-fat-induced injury of neonatal rat cardiomyocytes through increasing mitochondrial biogenesis and function

作　　者：王瀚[1] 闫文俊[1] 周芬[1] 张立剑[1] 李文婷[1] 刘佩林[1] 陶凌[1] 

机构地区：[1]第四军医大学西京医院心内科,陕西西安710032

出　　处：《心脏杂志》2013年第2期146-150,共5页Chinese Heart Journal

基　　金：国家自然科学基金项目资助(81170186;81225001)

摘　　要：目的:观察脂联素(APN)是否通过增强线粒体生物合成和功能减轻高糖/高脂所致心肌细胞损伤。方法:分离培养SD乳鼠的心肌细胞,培养3 d后分为3组:即对照组(培养基中含5 mmol/L葡萄糖和20 mmol/L甘露醇)、高糖/高脂组(培养基中含25 mmol/L葡萄糖和500μmol/L软脂酸钠,培养18 h)及高糖/高脂+APN组(培养基中含25 mmol/L葡萄糖,500μmol/L软脂酸钠和3μg/ml APN球状片段,培养18 h)。高糖/高脂处理后,检测转录因子Tfam mRNA的水平、细胞线粒体膜电位与心肌细胞的凋亡。结果:与对照组比较,高糖/高脂组Tfam mRNA的水平与线粒体膜电位均降低,细胞凋亡增加;APN处理可逆转上述作用。结论:高糖/高脂降低心肌线粒体生物合成和导致线粒体功能障碍;APN可通过增加线粒体的生物合成和功能而发挥对心肌保护的作用。AIM： To high -glucose/high-fat investigate whether adiponectin （APN） damage through increasing mitochondrial generates cardioprotection in response to biogenesis and functions. METHODS： Cultured neonatal rat ventricular myocytes （NRVM） were randomized into control group （5 mmol/L glu- cose and 20 mmol/L mannitol） , high-glucose/high-fat group （25 mmol/L glucose and 500 μmol/L sodi- um palmitate, 18 h incubation ） and high-glucose/high-fat ＋ APN group （25 mmol/L glucose, 500 μmol/L sodium palmitate and 3 μg/ml globular APN, 18 h incubation） after 3 days of normal culture. Ceils were harvested for evaluation of mitochondrial membrane potential and mRNA levels of Tfam after high-glucose/high-fat incubation. RESULTS： Compared with those in control group, both mitochondrial membrane potential and mRNA levels of Tram decreased and cell apoptosis increased in high-glucose/ high-fat group. However, these changes were reversed by APN. CONCLUSION： High-glucose/high-fat can lead to mitochondrial dysfunction in cardiomyocytes and APN can protect cardiomyocytes by increas- ing mitochondrial biogenesis and functions.

关 键 词：脂联素 心肌细胞 高糖 高脂 线粒体生物合成 线粒体功能 

分 类 号：R589.2[医药卫生—内分泌]