增龄相关性听力损失大鼠耳蜗TMPRSS3和ENaC-α蛋白的表达  

Expression of TMPRSS3 and ENaC-α in the cochlea of rats with age-related hearing loss

作　　者：汪芹[1,2] 葛圣雷[1,2] 伍伟景[1,2] 李友忠[1,2] 

机构地区：[1]中南大学湘雅二医院耳鼻咽喉头颈外科 [2]中南大学耳科研究所,湖南长沙410011

出　　处：《中国耳鼻咽喉颅底外科杂志》2013年第2期93-97,共5页Chinese Journal of Otorhinolaryngology-skull Base Surgery

基　　金：国家自然科学基金(30700940)

摘　　要：目的通过检测增龄相关性听力损失SD大鼠耳蜗中跨膜丝氨酸蛋白酶3(transmembrane protease,serine 3,TMPRSS3)、表皮钠通道α(epithelial sodium channel-a,ENaC-α)蛋白的表达,初步探讨其在增龄相关性听力损失中的作用。方法选用3、12及24月龄SD大鼠各30只,应用免疫组化、Western blot技术检测各组大鼠耳蜗中TMPRSS3、ENaC-α蛋白表达水平。结果随月龄增大,大鼠ABR阈值逐渐升高,耳蜗TMPRSS3、ENaC-α蛋白表达水平逐渐降低,差异具有统计学意义(P<0.01)。结论增龄相关性听力损失大鼠的耳蜗TMPRSS3、ENaC-α蛋白随月龄增大而降低。ENaC-α蛋白的变化与TMPRSS3呈相关性,提示TMPRSS3可能通过调节ENaC-α蛋白而起作用。Objective To explore the role of TMPRSS3 and ENaC-α in sensorineural deafness via investigating the expression in the cochlea of SD rats with age-related hearing loss. Methods Rats used for age- related hearing loss were divided into three groups, for 3-month-old group, 12- month-old group and 24-month-old group. The auditory function was evaluated by evoked auditory brainstem responses （ABR）. The expression of TMPRSS3 and ENaC-α in the cochlea was detected by Paraffin-embedded immunohistochemistry and Western blot assay. Results ABR threshold increased and expression of TMPRSS3, ENaC-α decreased with aging in the cochlea of SD rats. Conclusions The expression of TMPRSS3 , ENaC-α decreases with aging in the cochlea of SD rats. The change of ENaC-α expression has some correlation with TMPRSS3 which indicates that TMPRSS3 may regulate the expression of ENaC-α.

关 键 词：增龄相关性听力损失 跨膜丝氨酸蛋白酶3 表皮钠通道α 耳蜗 

分 类 号：R764.43[医药卫生—耳鼻咽喉科] Q341[医药卫生—临床医学]