棕色脂肪组织特异性基因在抵抗肥胖中作用的研究  

Role of Brown Adipose Tissue Characteristic Genes in Resisting Obesity

作　　者：孙慧[1] 杨娜娜[1] 黄雪芳[1] 付妤[1] 侯晓华[1] 徐三平[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院消化内科,武汉430022

出　　处：《临床消化病杂志》2013年第1期3-9,共7页Chinese Journal of Clinical Gastroenterology

基　　金：国家自然科学基金课题;No.81000159

摘　　要：目的通过研究高脂饮食诱导肥胖与肥胖抵抗大鼠肩胛间棕色脂肪组织中UCP-1、PGC-1α、Dio-2的表达情况以及电针刺激对肥胖大鼠UCP-1、PGC-1α表达的影响,探讨棕色脂肪组织特异性基因在抵抗肥胖中的作用。方法 40只雄性SD大鼠,按体重随机分为高脂实验组(n=28)和基础对照组(n=12)。分别给予高脂饲料和基础饲料喂养。高脂饮食5周末,将高脂实验组大鼠体重大于基础对照组最大体重者归为饮食诱导肥胖大鼠(DIO),将高脂实验组大鼠体重低于对照组平均体重者归为饮食诱导肥胖抵抗大鼠(DIO-R)。从DIO随机取6只为肥胖组(n=6)与肥胖抵抗组(n=6)、基础对照组(n=6),比较各组大鼠体重、两种脂肪重水平,使用实时荧光定量PCR及Western blot方法比较三组肩胛间棕色脂肪组织中UCP-1、PGC-1α、Dio-2表达水平的差异。在DIO大鼠中再取10只随机分为:肥胖组(Ob组,n=5)、电针刺激组(EA组,n=5)与基础对照组(n=5)以基础饲料适应性喂养1周后,其中EA组选取足三里、三阴交给予电针刺激,每周3次,每次30 min,观察摄食量及体重变化。6周后使用实时荧光定量PCR及Westernblot方法比较3组大鼠棕色脂肪组织中UCP-1、PGC-1α表达水平的差异。结果 DIO组明显高于DIO-R组,DIO-R组大鼠肩胛间棕色脂肪组织重及Dio-2、PGC-1α、UCP-1mRNA表达水平明显高于DIO大鼠(P<0.05)。高脂组大鼠PGC-1α、Dio-2m RNA表达水平均低于对照组大鼠(P<0.05);DIO-R组大鼠UCP-1 mRNA表达水平高于对照组大鼠(P<0.05)。DIO大鼠UCP-1蛋白水平表达低于基础对照组及DIO-R大鼠(P<0.05)。电针刺激6周后,电针刺激组大鼠棕色脂肪组织中UCP-1、PGC-1αmRNA及蛋白水平表达均高于Ob组及对照组(P<0.05)。电针刺激组大鼠体重、摄食量低于肥胖组大鼠(P<0.05)。结论高脂饮食条件下,SD大鼠表现为明显的肥胖易感性差异。饮食诱导肥胖抵抗大鼠棕色脂肪组织重及特异性基因表达升高。Objective To study the brown adipose tissue（BAT） characteristic genes expression in diet-induced obesity-resistant（DIO-R） and diet-induced obesity（DIO） rats and to explore the effects of acupuncture on expression of characteristic genes in interscapular brown adipose tissue,elucidate mechanisms of acupuncture in slimming.Methods 40 male Sprague-Dawley（SD） rats were randomly divided into 2 groups：basic group（n=12） and high-fat group（n=28）,they were fed with basic diet and high-fat diet respectively for 5 weeks.DIO-R and DIO rats were selected according to their body weight,then the intake of diet and body fat contents were measured,the expression levels of BAT specific genes were determined by real-time PCR and western blot.Diet-induced obesity（DIO） rats were divided into 2 groups：obesity group（Ob,n=5）,acupuncture group（EA,n=5）.5 rats were selected randomly from basic group as control group.3 groups were all given basic diet for 1 week,and then acupuncture with electric on ＂Housanli＂（ST 36） and ＂Sanyinjiao＂（SP 6） of the EA group rats,with left and right interchange,30mins each time and 3 times a week for 6 weeks.The weight of rats,fat weight around the kidney,intake of diet and expression levels of BAT specific genes between 3 groups were compared.Results The results showed that the body weight,pararenal or epididymal white fat tissues in DIO-R rats were significantly lower than those of DIO rats（P＆lt;0.05）.Meanwhile,the interscapular BAT of DIO-R rats was higher compared to DIO rats（P＆lt;0.05）.The expression of UCP-1,PGC-1α and Dio-2 mRNA of interscapular BAT in DIO-R rats were higher than that of DIO rats（P＆lt;0.05）.The PGC-1α、Dio-2 mRNA levels of rats in high-fat diet group were lower compared to basic diet group,while UCP-1 mRNA levels were higher.（P＆lt;0.05）.The UCP-1 protein levels of BAT in DIO rats were lower than that of other two groups（P＆lt;0.05）.After 6 weeks of acupuncture,the expression of UCP-1 and PGC-1α mRNA

关 键 词：饮食诱导肥胖 棕色脂肪组织 电针刺激 UCP-1 PGC-1Α Dio-2 

分 类 号：R589.2[医药卫生—内分泌]