外源性硫化氢对急性坏死性胰腺炎的影响  

Effects of Exogenous Hydrogen Sulfide on Acute Necrotizing Pancreatitis

作　　者：张永[1] 李斌[1] 鲁彦[2] 程东升[2] 柴琛[1] 颉宝平[1] 曹农[1] 

机构地区：[1]兰州大学第一医院普外一科,甘肃兰州730000 [2]解放军第一医院检验科,甘肃兰州730030

出　　处：《中国普外基础与临床杂志》2013年第4期385-388,共4页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金资助项目(项目编号:30700266);中央高校基本科研业务费专项资金(项目编号:lzujbky-2010-146)~~

摘　　要：目的探讨硫化氢(H2S)对急性坏死性胰腺炎(ANP)的影响。方法 43只SD雄性大鼠按数字表法行完全随机分组:假手术组(n=4);ANP组(n=21),该组再均分为3、6及12 h 3个亚组(n=7);NaCl+ANP组(n=4);NaHS+ANP组(n=7);以及炔丙基甘氨酸(PAG)+ANP组(n=7)。通过胰胆管逆行注射5%牛磺胆酸钠制作ANP模型;NaCl+ANP组、NaHS+ANP组及PAG+ANP组大鼠均在模型制作术前1 h分别给予生理盐水、NaHS及PAG进行预处理。检测各组大鼠血清淀粉酶(AMY)、谷草转氨酶(AST)、谷丙转氨酶(ALT)、血尿素氮(BUN)及血肌酐(Cr)水平,并观察胰腺组织的病理组织学改变。结果 ANP组大鼠制模术后血清AMY、AST、ALT、BUN及Cr水平进行性升高。NaHS+ANP组大鼠血清AMY、AST、ALT、BUN及Cr水平高于NaCl+ANP组(P<0.05),胰腺组织的病理损伤也较NaCl+ANP组严重。PAG+ANP组大鼠血清AMY、AST、ALT、BUN及Cr水平则低于NaCl+ANP组(P<0.05),胰腺组织的病理损伤也较NaCl+ANP组轻。结论 ANP肝、肾及胰腺功能受损可能与H2S浓度升高有关,预防性给予H2S阻断剂PAG可改善肝、肾及胰腺功能,具有器官保护作用。Objective To explore the influences of hydrogen sulfide （HzS） on acute necrotizing pancreatitis （ANP）, Methods Forty-three SD male rats were grouped by random number table, and divided into five groups： tile sham group （n=4）, ANP group n=21, which was divided into 3 subgroups： 3, 6, and 12 hours group （n=7） , NaCl＋ANP group （n=4）, NaHS＋ANP group （n=7）, and PAG＋ANP group （n=7）. Models of ANP were formed by re^trograde cholangiopancreatography injection of 5% sodium taurocholate. The NaCI ＋ ANP group, NariS ＋ ANP group, and PAG ＋ ANP group rats were given pretreatmcnt of saline, NariS, or PAG at 1 hour before modeling respectively. The levels of serum amylase （AMY）, aspartate aminotransferase （AST）, alanine aminotransferase （ALT）, blood urea nitrogen （BUN）, and creatinine （Cr） were detected, and the pathological histological changes of pancreatic tissues were observed. Results The levels of serum AMY, AST, ALl＇, BUN, and Cr were increased in ANP group. The levels of serum AMY, AST, ALT, BUN, and Cr in the NariS ＋ ANP group were higher than those of NaC1 ＋ ANP group （P〈0. 05）, and the pathological damage of the pancreatic tissues was more serious in the NaHS＋ANP group. The levels of serum AMY, AST, ALT, BUN, and Cr in the PAG＋ANP group were lower than those of NaCI＋ANP group （P〈0. 05）, and the pathological damage of pancreatic tissues in the PAG＋ANP group was not so serious as in the： NaCl ＋ ANP group. Conclusions The impairment of liver, kidney, and pancreas function in ANP rats may be related to elevated H2S concentration. Prophylactic administration the PAG of H2S antagonist can improve the function of the liver, kidney, and pancreas, and have the effects of organ protection.

关 键 词：急性坏死性胰腺炎 硫化氢 肝脏 肾脏 胰腺 

分 类 号：R657.5[医药卫生—外科学]