四(4-三甲胺苯)卟啉抗原抗体的制备与抗体效价的测定  

Synthesis and Evaluation of Antigen Antibody Tetra (Tetra Trimethylamine Benzene) Porphyrin

作　　者：祁超[1] 刘淑清[2] 赵晓松[3] 赵文斌[1] 

机构地区：[1]中国人民解放军军需大学中心实验室,长春130062 [2]吉林大学化学系,长春130021 [3]吉林农业大学资源与环境学院,长春130118

出　　处：《吉林农业大学学报》2000年第3期78-80,共3页Journal of Jilin Agricultural University

基　　金：国家自然科学基金! (2 970 10 0 5 )

摘　　要：利用邻硝基苯甲醛和吡咯合成H2 TNO2 PP ,再还原成H2 TamPP ,最后合成四 (α ,α ,α ,α O 三甲胺苯 )卟啉。用它免疫BALB/C小鼠 ,酶联免疫吸附法测定抗体效价达 6 40。表明该半抗原加载体 。Tetra (α, α, α, α O trimethylamine benzene) porphyrin was synthesized from H 2TamPP, product from reduction of H 2T NO 2 PP which was synthesized from O nitrobenzaldehyde and pyrrole. Tetra (α, α, α, α O trimethylamine benzene) porphyrin was used to immunize BALB/C mice. The antibody value determined through ELISA was 640, which indicated that the semiantigen might induce the formation of antibody without carrier.

关 键 词：合成 半抗原 卟啉 酶联免疫吸附法 

分 类 号：R446.61[医药卫生—诊断学]