芹菜子的薄层色谱鉴别及水分、灰分、浸出物测定  

Determination of moisture,ash,extractives and TLC identification of the Celery Seeds

作　　者：李娟[1] 艾买提江.阿依甫别克 陈丽[3] 阿吉艾克拜尔.艾萨 

机构地区：[1]新疆医科大学研究生院,乌鲁木齐830011 [2]新疆维吾尔自治区食品药品检验所,乌鲁木齐830004 [3]中国科学院新疆理化技术研究所,乌鲁木齐830011

出　　处：《新疆医科大学学报》2013年第4期456-459,共4页Journal of Xinjiang Medical University

基　　金：国家药品(维药)标准提高项目(516)

摘　　要：目的分析芹菜子的薄层鉴别及水分、灰分、浸出物,为制定芹菜子药材质量标准提供科学依据。方法收集不同批次的芹菜子药材,以芹菜素和木犀草素为指标,采用薄层色谱法建立了薄层鉴别,并按《中国药典》(2010年版一部)中相关规定,对该药材中水分、灰分和浸出物醇浸出物等项目进行测定。结果薄层鉴别结果显示,供试品色谱图中芹菜素和木犀草素的斑点清晰,分离度较好;该药材中水分<7.5%,总灰分<15.0%,酸不溶性灰分<1.5%;采用热浸法,其醇溶性浸出物>20.0%。结论该组方法简便、准确、重复性好,为建立芹菜子药材质量标准提供了准确可靠的实验依据。Objective To develop a scientific method for the quality standards of Celery Seeds.Methods This study collected from different batches of Celery Seeds,thin-layer chromatography(TLC) identification method was carried out by using apigenin and luteolin as an indicator,and determined moisture,ash,water-soluble extractives,ethanol-soluble extractives,addording to The Pharmacopoeia of the PRC.Results The TLC identification method was a specific method with good resolution.The moisture was less than 7.5%,total ash was not more than 15.0%,acid insoluble ash was not cross over 1.5%,ethanol-soluble extractives were not less than 20.0%.Conclusions This method was simple,accurate and reproducible.The quality standards can provide a reliable experimental basis for Celery Seeds.

关 键 词：芹菜子 质量标准 薄层色谱 浸出物 

分 类 号：R927.2[医药卫生—药学]