白花丹素衍生物Pln-7-co(oAc)体外抗肿瘤作用研究  

In vitro Anti-tumor Effect of Plumbagin Derivative Pln-7-co(oAc)

作　　者：彭艳[1,2] 蔡乐靖[1] 周远华[1] 黎智[1] 刘仁路[1] 仲辉[2] 张国海[2] 

机构地区：[1]广西师范大学生命科学学院,广西桂林541004 [2]广西师范大学化学化工学院,广西桂林541004

出　　处：《广西师范大学学报（自然科学版）》2013年第1期67-71,共5页Journal of Guangxi Normal University:Natural Science Edition

基　　金：973计划前期研究专项(2011CB512005);广西自然科学基金研究生创新项目(2011106020710M55);药用资源化学与药物分子工程教育部重点实验室课题(CMEMR2012-A12;CMEMR2012-A15)

摘　　要：本文以人肺癌NCI-H460、A549,肝癌BEL-7404,胃癌MCG-803细胞株以及正常肝细胞HL-7702作为受试细胞,分别采用MTT比色法、qPCR技术检测白花丹素衍生物(Pln-7-co(oAc))对细胞增殖的抑制作用以及对抑癌基因LRRC3B mRNA表达量的影响。结果显示,在10μmol/L药物的作用下Pln-7-co(oAc)对NCI-H460细胞增殖的抑制率达到86.68%±2.89%,明显高于A549(59.56%±9.00%),肝癌BEL-7404(-7.24%±2.93%),胃癌MCG-803(15.27%±5.87%)。在IC50测试实验中,Pln-7-co(oAc)对NCI-H460细胞株的增殖抑制作用呈现很好的剂量依赖关系,其IC50值低至(4.85±0.61)μmol/L,明显优于阳性药物易瑞沙Iressa(20.17±2.15)μmol/L,LRRC3B mRNA的相对表达量明显升高。The anti-tumor effect in vitro of plumbagin derivative （Pln-7-co（oAc）） is investigated. Human lung cancer cell lines （NCI-H460,A549）,human liver cancer cell line BEL-7404,human gastric cancer cell line MCG-803 and human normal liver cell line HL-7702 were served as the tested cells. The cell proliferation inhibition rates of Pln-7-co （oAc） were detected by MTT colorimetric assay. The relative expression of LRRC3B mRNA after Pln-7-co （oAc） treated was tested by qPCR. The inhibition rate of Pln- 7-co（oAc） on NCI-H460 cells （86.68%±2.89%） was much higher than that on A549 cells （59.56%± 9.00%）,BEL-7404 cells （-7. 24%±2.93%）,MCG-803 cells （15. 27%±5.87%） at a 10 μmol/L concentration. In IC50 test,the inhibition rate of Pln-7-co（oAc） on NCI-H460 cells showed a good dose de- pendency with IC50 value as low as （4.85±0.61）μmol/L,which was significantly better than the positive drug Iressa （20. 17±2.15）μmol/L. The relative expression of LRRC3B mRNA of Pln-7-co （oAc） group markedly increased compared with that of the control group.

关 键 词：NCI-H460 白花丹素衍生物 细胞增殖 抑癌基因LRRC3B 

分 类 号：R284.3[医药卫生—中药学] R979.1[医药卫生—中医学]