一株产海因酶菌种的筛选与鉴定  

Screening and Identification of a Strain of Hydantoinase-producing Microorganism

作　　者：王培[1,2] 曹建华[1] 宋德贵[2] 辜澜涛[2] 董研玲[3] 

机构地区：[1]中国地质科学院岩溶地质研究所国土资源部岩溶动力学重点实验室,广西桂林541004 [2]广西师范大学生命科学学院,广西桂林541004 [3]武汉生物工程学院,湖北武汉430415

出　　处：《广西师范大学学报（自然科学版）》2013年第1期119-124,共6页Journal of Guangxi Normal University:Natural Science Edition

基　　金：中国岩溶碳汇过程与效应(水[2011]矿评01-15-02);国土资源部、广西壮族自治区岩溶动力学重点实验室开放课题、主任基金(KDL2012-04)

摘　　要：通过对土壤中微生物以单一海因类似物作为唯一氮源富集、分离和纯化,利用双层琼脂法筛选获得产海因酶菌株,以基因工程手段提取其DNA,并进行特异性的16S rDNA测序,最终利用Blastn工具将其基因序列与GenBank数据库中的进行序列比对。研究表明:该菌与Bacillus megateriumQM B1551(NC004604)序列相似性达到98%,证明该菌为巨大芽孢杆菌。Through gathering,isolation and purification ,a strain of Hydantoinase-producing microorganisms from soil microbial based on single hydantoin analogue was taken as the onlynitrogen source. The Hydantoinase-producing microorganisms was screened by utilizing the Double agar-agar method. Then,it DNA was extracted by the means of genetic engineering method,and sequence of its specificity 16S rD- NA was tested. Lastly,a comparison its gene sequence to GenBank database by Blastn tool. Research re- sult shows ：this microorganism has 98 % similarity with Bacillus megaterium QM B1551 （NC _ 004604）, which proved that it is Bacillus megaterium.

关 键 词：海因酶 双层琼脂法 16SrDNA 巨大芽孢杆菌 

分 类 号：Q93-331[生物学—微生物学]