荧光增白剂致中华仓鼠肺细胞染色体畸变研究  

Chromosome aberration of Chinese hamster lung cells induced by fluorescent brightener

作　　者：蒋伟[1] 陈相[1] 缪文彬[1] 朱洪坤[1] 

机构地区：[1]上海出入境检验检疫局,上海200135

出　　处：《职业卫生与应急救援》2013年第2期71-73,共3页Occupational Health and Emergency Rescue

基　　金：国家质检总局科技计划项目(2011IK042)

摘　　要：[目的]荧光增白剂是国家安全生产监督管理总局2011年颁布的《职业病危害因素分类表》中定性的化学毒物,本研究探讨荧光增白剂染毒对体外哺乳动物细胞的染色体的影响,为荧光增白剂遗传损伤机制的研究提供科学依据。[方法]使用不同浓度的荧光增白剂28处理中国仓鼠肺细胞,通过体外哺乳动物染色体畸变实验检测细胞的染色体畸变程度。[结果]在有代谢活化系统条件下,各剂量染毒组结构畸变细胞率与阴性对照组相比,差异无统计学意义。[结论]本研究未显示荧光增白剂28造成体外哺乳动物细胞染色体损伤。[Objective] Fluorescent brightener is one of chemicals listed in ＂Classification Chart of Occupational Hazardous ＂announced by SAWS. In order to understand the mechanism of genetic damage, the chromosome damage induced by fluorescent brightener on CHL （Chinese hamster lung） cells was studied. [ Methods ] CHL cells were treated with different concentrations of fluorescent brightener 28 in vitro and chromosome aberration of treated CHL cells was detected. [Rosults] Under the testing conditions with an exogenous source of metabolic activation system （$9）, there was no significant increasing percentage of cells with structural aberrations in treated groups, compared with negative control. [ Conclusion ] Fluorescent brightener 28 may not induce chromosome damage in cultured mammalian cells.

关 键 词：荧光增白剂 体外哺乳动物细胞染色体畸变实验 遗传物质损伤 

分 类 号：R114[医药卫生—卫生毒理学]