高效液相色谱-质谱法检测Wistar大鼠主动脉6-keto-PGF_(1α)的生成  

Determination of 6-keto-PGF_(1α) in Rat Aortas with High Performance Liquid Chromatography-Mass Spectrometry

作　　者：张英展[1] 祝宁侠[1] 周应毕[1] 刘斌[1] 

机构地区：[1]汕头大学医学院心血管研究中心,广东汕头515041

出　　处：《汕头大学医学院学报》2013年第1期21-22,39,共3页Journal of Shantou University Medical College

基　　金：国家自然科学基金资助项目(81000111)

摘　　要：目的:建立大鼠主动脉6-keto-PGF1α的高效液相色谱-质谱(HPLC-MS)检测方法,以反映前列环素(PGI2)的产生水平。方法:建立用HPLC-MS同时检测环氧化酶(COX)代谢通路的5种前列腺素类产物的方法和Wistar大鼠主动脉产生的6-keto-PGF1α水平。结果:HPLC-MS检测法能同时检测COX代谢通路的5种PGI2产物和大鼠主动脉产生的6-keto-PGF1α;加入内皮受体激动剂乙酰胆碱后6-keto-PGF1α的生成明显升高。结论:成功建立用HPLC-MS检测Wistar大鼠主动脉所生成6-keto-PGF1α的方法。Objective：To detect the synthesis level of prostacyclin（PGI2）in rat aortas with the established method of high performance liquid chromatography-mass spectrometry（HPLC-MS）.Methods： HPLC-MS was established to analyze the five kinds of prostaglandin productions derived from cyclooxygenase（COX）mediated metabolism.The PGI2 metabolite 6-keto-PGF1α of aortas isolated from Wistar rats was analyzed.Results： HPLC-MS could be applied to detect the five prostaglandin productions derived from the COX-mediating pathway.The PGI2 metabolite 6-keto-PGF1α produced by Wistar rat aortas was well determined by HPLC-MS.The amount of 6-keto-PGF1α was obviously augmented when stimulated with the endothelial receptor agonist acetylcholine.Conclusion： The method of HPLC-MS is well established to detect 6-keto-PGF1α produced by aortas from Wistar rats.

关 键 词：前列环素 环氧化酶 主动脉 高效液相色谱-质谱 

分 类 号：R33-33[医药卫生—人体生理学]