双向电泳图像分析软件  

Evaluation of 2D Image Analysis Software

作　　者：贾宇峰[1] 刘少君[1] 郭尧君[2] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850 [2]中国科学院生物物理研究所,北京100101

出　　处：《现代科学仪器》2000年第5期20-23,共4页Modern Scientific Instruments

基　　金：国家自然科学基金资助项目 !No .3992 80 1 5

摘　　要：本文分别使用ImageMaster 2DElite 3 0 1和PDQuest 6 0对PC12细胞蛋白样品双向电泳结果进行了初步分析 ,讨论了软件的图像扫描、点检测、背景消除、匹配、结果报告和数据分析功能。并且对新一代的 2D图像分析软件 ,ImageMaster 2DElite 3 0 1、PDQuest 6 0和MelanieII&The 2D mapping of proteins extracted from PC12 cells were processed with ImageMaster 2D Elite 3 01 and PDQuest 6 0 respectively. Their representations in image acquirement, spot detection, background subtraction, match, report and database query, were also discussed. And The main features of the third_generation software for 2_DE analysis, ImageMaster 2D Elite 3 01, PDQuest 6 0, and Melanie II & 3 were compared.

关 键 词：双向电泳 图像分析软件 蛋白质组 

分 类 号：Q51[生物学—生物化学] TP317.4[自动化与计算机技术—计算机软件与理论]