尿酸在纳米金-聚番红花红修饰电极上的电化学行为及修饰电极的应用研究  

Electrochemical Behavior of Uric Acid at Poly(safranine T) and Gold Nanoparticles Composite Modified Glassy Carbon Electrode and Application of the Modified Electrode

作　　者：牛凌梅[1] 张玉娜[2] 连靠奇[1] 石红梅[1] 康维钧[1] 

机构地区：[1]河北医科大学公共卫生学院,石家庄050017 [2]河北医科大学第四医院内分泌科,石家庄050011

出　　处：《生命科学仪器》2013年第1期27-30,共4页Life Science Instruments

基　　金：国家自然科学基金(81172722;81273128)项目资助

摘　　要：本研究制备了纳米金-聚番红花红复合膜修饰的玻碳电极,并利用差分脉冲法(DPV)研究了尿酸在此修饰电极上的电化学行为。结果表明纳米金-聚番红花红复合膜对于尿酸的氧化能够起到明显的电催化作用。在优化条件下,尿酸的氧化峰电流与其浓度在1.0×10^(-6)～1.0×10^(-4)mol/L范围内呈良好的线性关系,检出限为6.0×10^(-7)mol/L。该方法快速,准确,将聚纳米金-番红花红复合膜修饰电极用于体液中尿酸的测定,回收率在95.0%～100%之间。In this study, glassy carbon electrode modified with gold nanoparticles - poly safranin composite film was prepared, and the electrochemical behavior of uric acid in this modified electrode was studied by differential pulse method （DPV）. The results indicated that the gold nanoparticles -poly safranin composite membrane showed a significant electrical catalysis for the oxidation of uric acid. Under optimized conditions, a good linear relationship was detected between the oxidation peak current and the concentration when the concentration of uric acid was within 1.0×10^-6～1.0×10^-4mol/L. The detection limit was 10^-7 mol/L. The method was proved to be rapid and accurate. This modified electrode was used to detect the uric acid in body fluids, and the recovery was between 95.0% and 100%.

关 键 词：番红花红 纳米金 玻碳电极 尿酸 

分 类 号：O657.1[理学—分析化学]