新生大鼠海马神经干细胞的离体培养及细胞连接的超微结构观察  

Culture of neonatal rats hippocampal neural stem cells and cell junction ultrastructure observation

作　　者：董富兴[1] 胡莹莹[2] 刘亚萍[3] 于红丽[1] 赵玉 段现花[2] 董红燕[1] 徐铁军[2] 

机构地区：[1]徐州医学院神经生物学研究中心电镜室,江苏徐州221004 [2]徐州医学院人体解剖与组织胚胎学教研室,江苏徐州221004 [3]徐州医学院形态学实验教学中心,江苏徐州221004 [4]伊利诺依理工大学生命科学学院,美国芝加哥伊利诺依60616

出　　处：《重庆医学》2013年第11期1201-1203,1206,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(81171141);江苏省高校自然科学基础研究计划项目(07KJB310119);徐州医学院科研课题(2010KJ18;2012KJ09)

摘　　要：目的观察体外培养的新生大鼠海马神经干细胞球的超微结构特点。方法取处于指数期的原代培养7d的新生大鼠海马神经干细胞,常规制备透射电镜样品后,用日立H-600透射电镜观察并摄片。结果光镜下神经干细胞呈克隆球散在悬浮于培养基中,Nestin免疫荧光阳性;电镜下观察到神经干细胞相互间排列比较松散、核浆比例高,细胞间胞膜增厚,形成特化的结构,有的胞膜上可见胞吞/胞吐小泡。结论体外培养的新生大鼠海马神经干细胞间的细胞连接有间隙连接样结构,并可出现胞吞/胞吐功能,提示细胞间可进行相互交流,传递化学递质或电兴奋活动。Objective To observe the ultrastructural characteristics of neural stem cells(NSCs) from neonatal rats hippocampal.Methods Taking the primary cultured neonatal rat hippocampus NSCs which were on the exponential phase and cultured 7 days,then we observe the ultrastructure of them and take photos with Hitachi H-600 transmission electron microscope after prepared for routine transmission electron microscope samples.Results NSCs were scattered in the suspension medium under Light microscopy,and were Nestin-positive;under electron microscopy,we can see the NSCs were loosely arranged with each other,high karyoplasmic ratio,cell membrane thickening,formation of special structure and some endocytosis/exocytosis of vesicles were visible on the membrane.Conclusion There were gap junction-liked structure of the cell connection in cultured NSCs in vitro and endocytosis/exocytosis appeared which was suggesting that NSCs could communicate and pass chemical transmitters or electrical activity with each other.

关 键 词：海马 神经干细胞 细胞连接 超微结构 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]