错配修复基因hMSH2和hMLH1在结直肠癌组织中的表达及意义  

Expression of Mismatch Repair Gene hMLH1 and hMSH2 in Colorectal Carcinoma and Its Significance

作　　者：张苗尊[1] 章维[2] 

机构地区：[1]宁波大学医学院附属鄞州医院,浙江宁波315040 [2]宁波市第二医院,浙江宁波315010

出　　处：《中国肿瘤》2013年第4期308-311,共4页China Cancer

摘　　要：[目的]评价错配修复基因hMLH1和hMSH2在结直肠癌中的表达及意义。[方法]经病理学确诊的结直肠癌手术切除标本85例,术前均未接受过放疗或化疗,免疫组化检测hMLH1和hMSH2蛋白表达情况。[结果]85例结直肠癌组中,hMSH2缺失率为69.4%(59/85),hMLH1蛋白缺失率为78.8%(67/85)。hMSH2蛋白缺失率在T1、T2、T3和T4期结直肠肿瘤中的缺失率分别为50.0%、43.8%、71.1%和82.8%,随T分期增加而增加(χ2=11.037,P=0.012)。有淋巴结转移者的hMSH2蛋白缺失率达88.57%(31/35),而无淋巴结转移患者的hMSH2蛋白缺失率为56.0%(28/50)(χ2=10.287,P=0.001)。hMLH1蛋白缺失率与肿瘤分化程度和T分期相关,但与性别、年龄、肿瘤部位、N分期和M分期均无关。[结论]在结直肠癌组织中存在hMSH2和hMLH1蛋白缺失,且hMSH2和hMLH1蛋白缺失与肿瘤T分期和分化程度相关。检测hMLH1和hMSH2蛋白表达对预后判断有一定的参考价值。[Purpose] To investigate the expression of mismatch repair gene human mutl homolog 1 （hMLHI） and human mutl homolog 2 （hMSH2） in colorectal carcinoma and its significance. [Meth- ods] A total of 85 pathologically proved patients with colorectal carcinoma undergoing surgical re- section were included in the study. All the patients did not undergo preoperative chemotherapy or radiotherapy. The expression of hMLH1 and hMSH2 protein was detected by immunohistochemical method. [Resuhsl The loss rate of hMSH2 and hMLH1 expression in cancer tissue was 69.4%（59/ 85）,78.8%（67/85） respectively. The loss rate of hMSH2 expression in patients with T1,T2,T3,T4 was 50.0%,43.8% ,71.1% and 82.8% respectively. The loss rate of hMSH2 expression increased with T stage increasing（χ2=11.037 ,P=0.012）. The loss rate of hMSH2 expression in patients with lymph node metastases was 88.57% （31/35） ,but 56.0% （28/50）in patients without lymph node metastases with significant difference（χ2=10.287, P=0.001）. The loss rate of hMLH1 expression was correlated to tumor differentiation and T stage,but not correlated to gender,age,tumor site, N stage and M stage. [ Con- clusion] Loss expression of hMLH-1 and hMSH-2 protein exists in colorectal carcinoma. Expression loss of hMLH-1 and hMSH-2 protein correlates to tumor differentiation and T stage. Combined de- tection of hMLH-1 and hMSH-2 expression might be useful for evaluating prognosis of eolorectal cancer.

关 键 词：肠肿瘤 错配修复基因 HMLH1 HMSH2 免疫组化 

分 类 号：P753.3[交通运输工程—港口、海岸及近海工程]