黑龙江省主栽大豆品种脂肪含量分析及SSR分子标记辅助鉴定  

Analysis of Oil Content Underlying Major Soybean Cultivars of Heilongjiang Province and SSR Molecular Marker-assisted Identification

作　　者：孙梦阳[1] 王艳[1] 武林[1] 韩英鹏[1] 赵雪[1] 滕卫丽[1] 王桂玲[1] 李文滨[1] 

机构地区：[1]大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室/东北农业大学大豆研究所,黑龙江哈尔滨150030

出　　处：《大豆科学》2013年第2期143-148,共6页Soybean Science

基　　金：农业部大豆产业技术体系(CARS-04-PS04);国家"十二五"科技支撑计划(2011BAD35B06);国家重点基础研究发展计划"973计划"(2012CB126311);国家自然科学基金(31201227);中国博士后项目(20110491024);黑龙江省博士后项目(LBH11220);黑龙江省骨干教师项目(1252G014)

摘　　要：以黑龙江省92个主栽大豆品种(系)为研究对象,分别对其在2010年和2011年的脂肪含量进行分析,发掘与脂肪含量紧密连锁的SSR标记,用以辅助鉴定黑龙江省主栽大豆品种的脂肪含量。结果表明:供试材料脂肪含量变异范围2010年为17.10%～23.14%,2011年为17.70%～23.04%,两年平均为17.40%～23.09%。结合SSR数据的品种系统进化树分析,可以将黑龙江省主栽大豆品种脂肪含量总体上分成大于20%和小于20%两类。此外,鉴别出2个与脂肪含量相关的SSR标记(Satt428-900、Satt502-150),标记指数与脂肪含量显著相关(相关系数分别为-0.41*和0.41*)。A total of 92 major soybean cuhivars（lines） of Heilongjiang province ,which were analyzed for the oil content in year of 2010 and 2011 respectively,were chosen as the object to explore SSR markers associated with oil content in order to identify the oil content of the cuhivars（lines）. Analysis of variance underlying oil content showed that the range of variation of tested materials for oil content was 17.1% -23.14% in 2010 and 17.7% -23.04% in 2011, and the average was 17.4% -23.09%. The analysis of dendrogram of the cuhivars combined with SSR data could roughly define the major cultivars of Heilongjiang province into two class with 20% as the border of oil content. Moreover, two SSR markers （ Satt428-900, SattS02-150）, of which the label index showed significantly correlation with oil content with -0.41 ＊ and 0.41 ＊ as correlation coefficient respective- ly, were significantly correlated with oil content.

关 键 词：黑龙江省 主栽大豆品种(系) 脂肪含量 SSR标记辅助鉴定 相关分析 

分 类 号：S565.1[农业科学—作物学]